2ixy
From Proteopedia
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'''SOLUTION STRUCTURE OF THE APICAL STEM-LOOP OF THE HUMAN HEPATITIS B VIRUS ENCAPSIDATION SIGNAL''' | '''SOLUTION STRUCTURE OF THE APICAL STEM-LOOP OF THE HUMAN HEPATITIS B VIRUS ENCAPSIDATION SIGNAL''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IXY is a [[Single protein]] structure | + | 2IXY is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IXY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Wijmenga, S S.]] | [[Category: Wijmenga, S S.]] | ||
[[Category: Zdunek, J.]] | [[Category: Zdunek, J.]] | ||
| - | [[Category: | + | [[Category: Hbv]] |
| - | [[Category: | + | [[Category: Pseudo triloop]] |
| - | [[Category: | + | [[Category: Rdc]] |
| - | [[Category: | + | [[Category: Rna]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:03:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:03, 4 May 2008
SOLUTION STRUCTURE OF THE APICAL STEM-LOOP OF THE HUMAN HEPATITIS B VIRUS ENCAPSIDATION SIGNAL
Overview
Hepatitis B virus (HBV) replication is initiated by HBV RT binding to the highly conserved encapsidation signal, epsilon, at the 5' end of the RNA pregenome. Epsilon contains an apical stem-loop, whose residues are either totally conserved or show rare non-disruptive mutations. Here we present the structure of the apical stem-loop based on NOE, RDC and (1)H chemical shift NMR data. The (1)H chemical shifts proved to be crucial to define the loop conformation. The loop sequence 5'-CUGUGC-3' folds into a UGU triloop with a CG closing base pair and a bulged out C and hence forms a pseudo-triloop, a proposed protein recognition motif. In the UGU loop conformations most consistent with experimental data, the guanine nucleobase is located on the minor groove face and the two uracil bases on the major groove face. The underlying helix is disrupted by a conserved non-paired U bulge. This U bulge adopts multiple conformations, with the nucleobase being located either in the major groove or partially intercalated in the helix from the minor groove side, and bends the helical stem. The pseudo-triloop motif, together with the U bulge, may represent important anchor points for the initial recognition of epsilon by the viral RT.
About this Structure
2IXY is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Solution structure of the apical stem-loop of the human hepatitis B virus encapsidation signal., Flodell S, Petersen M, Girard F, Zdunek J, Kidd-Ljunggren K, Schleucher J, Wijmenga S, Nucleic Acids Res. 2006;34(16):4449-57. Epub 2006 Aug 31. PMID:16945960 Page seeded by OCA on Sun May 4 08:03:29 2008
Categories: Single protein | Flodell, S. | Girard, F. | Kidd-Ljunggren, K. | Petersen, M. | Schleucher, J. | Wijmenga, S S. | Zdunek, J. | Hbv | Pseudo triloop | Rdc | Rna
