2iyb
From Proteopedia
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'''STRUCTURE OF COMPLEX BETWEEN THE 3RD LIM DOMAIN OF TES AND THE EVH1 DOMAIN OF MENA''' | '''STRUCTURE OF COMPLEX BETWEEN THE 3RD LIM DOMAIN OF TES AND THE EVH1 DOMAIN OF MENA''' | ||
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[[Category: Briggs, D C.]] | [[Category: Briggs, D C.]] | ||
[[Category: Mcdonald, N Q.]] | [[Category: Mcdonald, N Q.]] | ||
| - | [[Category: | + | [[Category: Actin-binding]] |
| - | [[Category: | + | [[Category: Alternative splicing]] |
| - | [[Category: | + | [[Category: Coiled coil]] |
| - | [[Category: | + | [[Category: Cytoskeleton]] |
| - | [[Category: | + | [[Category: Lim domain]] |
| - | [[Category: | + | [[Category: Metal-binding]] |
| - | [[Category: | + | [[Category: Phosphorylation]] |
| - | [[Category: | + | [[Category: Sh3-binding]] |
| - | [[Category: | + | [[Category: Tumour supressor lim domain evh1 domain cell motility]] |
| - | [[Category: | + | [[Category: Zinc]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:04:27 2008'' | |
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Revision as of 05:04, 4 May 2008
STRUCTURE OF COMPLEX BETWEEN THE 3RD LIM DOMAIN OF TES AND THE EVH1 DOMAIN OF MENA
Overview
The intracellular targeting of Ena/VASP family members is achieved via the interaction of their EVH1 domain with FPPPP sequence motifs found in a variety of cytoskeletal proteins, including lamellipodin, vinculin, and zyxin. Here we show that the LIM3 domain of Tes, which lacks the FPPPP motif, binds to the EVH1 domain of Mena, but not to those of VASP or Evl. The structure of the LIM3:EVH1 complex reveals that Tes occludes the FPPPP-binding site and competes with FPPPP-containing proteins for EVH1 binding. Structure-based gain-of-function experiments define the molecular basis for the specificity of the Tes-Mena interaction. Consistent with in vitro observations, the LIM3 domain displaces Mena, but not VASP, from the leading edge and focal adhesions. It also regulates cell migration through a Mena-dependent mechanism. Our observations identify Tes as an atypical EVH1 binding partner and a regulator specific to a single Ena/VASP family member.
About this Structure
2IYB is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Tes, a specific Mena interacting partner, breaks the rules for EVH1 binding., Boeda B, Briggs DC, Higgins T, Garvalov BK, Fadden AJ, McDonald NQ, Way M, Mol Cell. 2007 Dec 28;28(6):1071-82. PMID:18158903 Page seeded by OCA on Sun May 4 08:04:27 2008
