2iyn

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[[Image:2iyn.gif|left|200px]]
[[Image:2iyn.gif|left|200px]]
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{{Structure
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|PDB= 2iyn |SIZE=350|CAPTION= <scene name='initialview01'>2iyn</scene>, resolution 2.08&Aring;
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The line below this paragraph, containing "STRUCTURE_2iyn", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+C'>AC1</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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|ACTIVITY=
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{{STRUCTURE_2iyn| PDB=2iyn | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iyn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iyn OCA], [http://www.ebi.ac.uk/pdbsum/2iyn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iyn RCSB]</span>
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'''THE CO-FACTOR-INDUCED PRE-ACTIVE CONFORMATION IN PHOB'''
'''THE CO-FACTOR-INDUCED PRE-ACTIVE CONFORMATION IN PHOB'''
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[[Category: Gomis-Ruth, F X.]]
[[Category: Gomis-Ruth, F X.]]
[[Category: Sola, M.]]
[[Category: Sola, M.]]
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[[Category: activation of the pho regulon]]
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[[Category: Activation of the pho regulon]]
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[[Category: activator]]
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[[Category: Activator]]
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[[Category: alpha/beta doubly wound fold]]
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[[Category: Alpha/beta doubly wound fold]]
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[[Category: dna- binding]]
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[[Category: Dna- binding]]
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[[Category: dna-binding]]
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[[Category: Dna-binding]]
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[[Category: gene regulation]]
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[[Category: Gene regulation]]
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[[Category: phosphate regulation]]
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[[Category: Phosphate regulation]]
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[[Category: phosphate transport]]
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[[Category: Phosphate transport]]
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[[Category: phosphorylation]]
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[[Category: Phosphorylation]]
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[[Category: sensory transduction]]
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[[Category: Sensory transduction]]
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[[Category: transcription]]
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[[Category: Transcription]]
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[[Category: transcription factor]]
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[[Category: Transcription factor]]
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[[Category: transcription regulation]]
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[[Category: Transcription regulation]]
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[[Category: transport]]
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[[Category: Transport]]
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[[Category: two-component regulatory system]]
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[[Category: Two-component regulatory system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:05:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:50:32 2008''
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Revision as of 05:05, 4 May 2008

Image:2iyn.gif

Template:STRUCTURE 2iyn

THE CO-FACTOR-INDUCED PRE-ACTIVE CONFORMATION IN PHOB


Overview

PhoB is an Escherichia coli transcription factor from a two-component signal transduction system that is sensitive to limiting environmental phosphate conditions. It consists of an N-terminal receiver domain (RD) and a C-terminal DNA-binding domain. The protein is activated upon phosphorylation at the RD, an event that depends on Mg(2+) binding. The structure of PhoB RD in complex with Mg(2+) is presented, which shows three protomers in the asymmetric unit that interact across two different surfaces. One association is symmetric and has been described as a non-active dimerization contact; the other involves the alpha4-beta5-alpha5 interface and recalls the contact found in activated PhoB. However, here this last interaction is not perfectly symmetric and helix alpha4, which in the activated molecule undergoes a helical shift, becomes strongly destabilized in one of the interacting monomers. All protomers bind the cation in a similar manner but, interestingly, at the prospective binding site for the phosphate moiety the side chains of either Glu88 (in helix alpha4) or Trp54 alternate and interact with active-site atoms. When Glu88 is inside the cavity, helix alpha4 is arranged similarly to the unliganded wild-type structure. However, if Trp54 is present, the helix loses its contacts with the active-site cavity and vanishes. Accordingly, the presence of Trp54 in the active site induces a flexible state in helix alpha4, potentially allowing a helical shift that phosphorylation would eventually stabilize.

About this Structure

2IYN is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The cofactor-induced pre-active conformation in PhoB., Sola M, Drew DL, Blanco AG, Gomis-Ruth FX, Coll M, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1046-57. Epub 2006, Aug 19. PMID:16929106 Page seeded by OCA on Sun May 4 08:05:04 2008

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OCA

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