2j0x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2j0x.gif|left|200px]]
[[Image:2j0x.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2j0x |SIZE=350|CAPTION= <scene name='initialview01'>2j0x</scene>, resolution 2.80&Aring;
+
The line below this paragraph, containing "STRUCTURE_2j0x", creates the "Structure Box" on the page.
-
|SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+A'>AC1</scene>
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_kinase Aspartate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.4 2.7.2.4] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2j0x| PDB=2j0x | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j0x OCA], [http://www.ebi.ac.uk/pdbsum/2j0x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j0x RCSB]</span>
+
-
}}
+
'''CRYSTAL STRUCTURE OF E. COLI ASPARTOKINASE III IN COMPLEX WITH LYSINE AND ASPARTATE (T-STATE)'''
'''CRYSTAL STRUCTURE OF E. COLI ASPARTOKINASE III IN COMPLEX WITH LYSINE AND ASPARTATE (T-STATE)'''
Line 31: Line 28:
[[Category: Ren, J.]]
[[Category: Ren, J.]]
[[Category: Stammers, D K.]]
[[Category: Stammers, D K.]]
-
[[Category: act domain]]
+
[[Category: Act domain]]
-
[[Category: allosteric regulation]]
+
[[Category: Allosteric regulation]]
-
[[Category: amino acid biosynthesis]]
+
[[Category: Amino acid biosynthesis]]
-
[[Category: amino-acid biosynthesis]]
+
[[Category: Amino-acid biosynthesis]]
-
[[Category: aspartate pathway]]
+
[[Category: Aspartate pathway]]
-
[[Category: aspartokinase]]
+
[[Category: Aspartokinase]]
-
[[Category: feedback inhibition]]
+
[[Category: Feedback inhibition]]
-
[[Category: kinase]]
+
[[Category: Kinase]]
-
[[Category: lysine]]
+
[[Category: Lysine]]
-
[[Category: lysine biosynthesis]]
+
[[Category: Lysine biosynthesis]]
-
[[Category: transferase]]
+
[[Category: Transferase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:11:21 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:51:34 2008''
+

Revision as of 05:11, 4 May 2008

Image:2j0x.gif

Template:STRUCTURE 2j0x

CRYSTAL STRUCTURE OF E. COLI ASPARTOKINASE III IN COMPLEX WITH LYSINE AND ASPARTATE (T-STATE)


Overview

Aspartokinase III (AKIII) from Escherichia coli catalyzes an initial commitment step of the aspartate pathway, giving biosynthesis of certain amino acids including lysine. We report crystal structures of AKIII in the inactive T-state with bound feedback allosteric inhibitor lysine and in the R-state with aspartate and ADP. The structures reveal an unusual configuration for the regulatory ACT domains, in which ACT2 is inserted into ACT1 rather than the expected tandem repeat. Comparison of R- and T-state AKIII indicates that binding of lysine to the regulatory ACT1 domain in R-state AKIII instigates a series of changes that release a "latch", the beta15-alphaK loop, from the catalytic domain, which in turn undergoes large rotational rearrangements, promoting tetramer formation and completion of the transition to the T-state. Lysine-induced allosteric transition in AKIII involves both destabilizing the R-state and stabilizing the T-state tetramer. Rearrangement of the catalytic domain blocks the ATP-binding site, which is therefore the structural basis for allosteric inhibition of AKIII by lysine.

About this Structure

2J0X is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structures of R- and T-state Escherichia coli aspartokinase III. Mechanisms of the allosteric transition and inhibition by lysine., Kotaka M, Ren J, Lockyer M, Hawkins AR, Stammers DK, J Biol Chem. 2006 Oct 20;281(42):31544-52. Epub 2006 Aug 12. PMID:16905770 Page seeded by OCA on Sun May 4 08:11:21 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j0x"
Personal tools