2j56
From Proteopedia
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[[Image:2j56.gif|left|200px]] | [[Image:2j56.gif|left|200px]] | ||
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'''X-RAY REDUCED PARACCOCUS DENITRIFICANS METHYLAMINE DEHYDROGENASE N-SEMIQUINONE IN COMPLEX WITH AMICYANIN.''' | '''X-RAY REDUCED PARACCOCUS DENITRIFICANS METHYLAMINE DEHYDROGENASE N-SEMIQUINONE IN COMPLEX WITH AMICYANIN.''' | ||
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[[Category: Pearson, A R.]] | [[Category: Pearson, A R.]] | ||
[[Category: Wilmot, C M.]] | [[Category: Wilmot, C M.]] | ||
- | [[Category: | + | [[Category: Copper]] |
- | [[Category: | + | [[Category: Electron transport]] |
- | [[Category: | + | [[Category: Metal-binding]] |
- | [[Category: | + | [[Category: Methylamine dehydrogenase single crystal microspectrophoto]] |
- | [[Category: | + | [[Category: Oxidoreductase]] |
- | [[Category: | + | [[Category: Periplasmic]] |
- | [[Category: | + | [[Category: Transport]] |
- | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:21:05 2008'' | |
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + |
Revision as of 05:21, 4 May 2008
X-RAY REDUCED PARACCOCUS DENITRIFICANS METHYLAMINE DEHYDROGENASE N-SEMIQUINONE IN COMPLEX WITH AMICYANIN.
Overview
X-ray exposure during crystallographic data collection can result in unintended redox changes in proteins containing functionally important redox centers. In order to directly monitor X-ray-derived redox changes in trapped oxidative half-reaction intermediates of Paracoccus denitrificans methylamine dehydrogenase, a commercially available single-crystal UV/Vis microspectrophotometer was installed on-line at the BioCARS beamline 14-BM-C at the Advanced Photon Source, Argonne, USA. Monitoring the redox state of the intermediates during X-ray exposure permitted the creation of a general multi-crystal data collection strategy to generate true structures of each redox intermediate.
About this Structure
2J56 is a Protein complex structure of sequences from Paracoccus denitrificans. Full crystallographic information is available from OCA.
Reference
Tracking X-ray-derived redox changes in crystals of a methylamine dehydrogenase/amicyanin complex using single-crystal UV/Vis microspectrophotometry., Pearson AR, Pahl R, Kovaleva EG, Davidson VL, Wilmot CM, J Synchrotron Radiat. 2007 Jan;14(Pt 1):92-8. Epub 2006 Dec 15. PMID:17211075 Page seeded by OCA on Sun May 4 08:21:05 2008