2j59

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[[Image:2j59.gif|left|200px]]
[[Image:2j59.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2j59 |SIZE=350|CAPTION= <scene name='initialview01'>2j59</scene>, resolution 2.1&Aring;
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The line below this paragraph, containing "STRUCTURE_2j59", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2j59| PDB=2j59 | SCENE= }}
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|RELATEDENTRY=[[1o3y|1O3Y]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j59 OCA], [http://www.ebi.ac.uk/pdbsum/2j59 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j59 RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF THE ARF1:ARHGAP21-ARFBD COMPLEX'''
'''CRYSTAL STRUCTURE OF THE ARF1:ARHGAP21-ARFBD COMPLEX'''
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[[Category: Menetrey, J.]]
[[Category: Menetrey, J.]]
[[Category: Perderiset, M.]]
[[Category: Perderiset, M.]]
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[[Category: actin organization]]
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[[Category: Actin organization]]
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[[Category: arf]]
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[[Category: Arf]]
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[[Category: arf1]]
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[[Category: Arf1]]
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[[Category: arfbd]]
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[[Category: Arfbd]]
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[[Category: arhgap21]]
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[[Category: Arhgap21]]
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[[Category: golgi apparatus]]
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[[Category: Golgi apparatus]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: myristate]]
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[[Category: Myristate]]
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[[Category: nucleotide-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: protein transport]]
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[[Category: Protein transport]]
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[[Category: rhogap protein]]
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[[Category: Rhogap protein]]
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[[Category: small gtp-binding protein]]
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[[Category: Small gtp-binding protein]]
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[[Category: transport]]
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[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:21:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:53:11 2008''
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Revision as of 05:21, 4 May 2008

Image:2j59.gif

Template:STRUCTURE 2j59

CRYSTAL STRUCTURE OF THE ARF1:ARHGAP21-ARFBD COMPLEX


Overview

ARHGAP21 is a Rho family GTPase-activating protein (RhoGAP) that controls the Arp2/3 complex and F-actin dynamics at the Golgi complex by regulating the activity of the small GTPase Cdc42. ARHGAP21 is recruited to the Golgi by binding to another small GTPase, ARF1. Here, we present the crystal structure of the activated GTP-bound form of ARF1 in a complex with the Arf-binding domain (ArfBD) of ARHGAP21 at 2.1 A resolution. We show that ArfBD comprises a PH domain adjoining a C-terminal alpha helix, and that ARF1 interacts with both of these structural motifs through its switch regions and triggers structural rearrangement of the PH domain. We used site-directed mutagenesis to confirm that both the PH domain and the helical motif are essential for the binding of ArfBD to ARF1 and for its recruitment to the Golgi. Our data demonstrate that two well-known small GTPase-binding motifs, the PH domain and the alpha helical motif, can combine to create a novel mode of binding to Arfs.

About this Structure

2J59 is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes., Menetrey J, Perderiset M, Cicolari J, Dubois T, Elkhatib N, El Khadali F, Franco M, Chavrier P, Houdusse A, EMBO J. 2007 Apr 4;26(7):1953-62. Epub 2007 Mar 8. PMID:17347647 Page seeded by OCA on Sun May 4 08:21:24 2008

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