This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2j6a
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2j6a.jpg|left|200px]] | [[Image:2j6a.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2j6a", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_2j6a| PDB=2j6a | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.''' | '''CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.''' | ||
| Line 32: | Line 29: | ||
[[Category: Tilbeurgh, H Van.]] | [[Category: Tilbeurgh, H Van.]] | ||
[[Category: Ulryck, N.]] | [[Category: Ulryck, N.]] | ||
| - | [[Category: | + | [[Category: Erf1]] |
| - | [[Category: | + | [[Category: Methyltransferase]] |
| - | [[Category: | + | [[Category: Nuclear protein]] |
| - | [[Category: | + | [[Category: Protein methylation]] |
| - | [[Category: | + | [[Category: Transferase]] |
| - | [[Category: | + | [[Category: Translation termination]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:24:15 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:24, 4 May 2008
CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.
Overview
Protein release factor eRF1 in Saccharomyces cerevisiae, in complex with eRF3 and GTP, is methylated on a functionally crucial Gln residue by the S-adenosylmethionine-dependent methyltransferase Ydr140w. Here we show that eRF1 methylation, in addition to these previously characterized components, requires a 15-kDa zinc-binding protein, Ynr046w. Co-expression in Escherichia coli of Ynr046w and Ydr140w allows the latter to be recovered in soluble form rather than as inclusion bodies, and the two proteins co-purify on nickel-nitrilotriacetic acid chromatography when Ydr140w alone carries a His tag. The crystal structure of Ynr046w has been determined to 1.7 A resolution. It comprises a zinc-binding domain built from both the N- and C-terminal sequences and an inserted domain, absent from bacterial and archaeal orthologs of the protein, composed of three alpha-helices. The active methyltransferase is the heterodimer Ydr140w.Ynr046w, but when alone, both in solution and in crystals, Ynr046w appears to be a homodimer. The Ynr046w eRF1 methyltransferase subunit is shared by the tRNA methyltransferase Trm11p and probably by two other enzymes containing a Rossman fold.
About this Structure
2J6A is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast., Heurgue-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH, J Biol Chem. 2006 Nov 24;281(47):36140-8. Epub 2006 Sep 28. PMID:17008308 Page seeded by OCA on Sun May 4 08:24:15 2008
