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2y8i

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Revision as of 06:35, 31 July 2019

Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2

Structural highlights

2y8i is a 1 chain structure with sequence from Dicdi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1mne, 1d1c, 1w9l, 1vom, 1mmn, 2aka, 2jhr, 1w9j, 2x9h, 1fmv, 1mma, 2xo8, 1mmg, 1w9i, 1w9k, 1mnd, 1jx2, 1q5g, 1d0y, 2xel, 2jj9, 1lvk, 1jwy, 1yv3, 1fmw, 1d0x, 1d1b, 1g8x, 1d1a, 1d0z, 1mmd, 2y0r, 2y9e
Activity:	Myosin ATPase, with EC number 3.6.4.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.

Publication Abstract from PubMed

The cold-sensitive single-residue mutation of glycine 680 in the reactive thiol region of Dictyostelium discoideum myosin-2 or the corresponding conserved glycine in other myosin isoforms has been reported to interfere with motor function. Here we present the x-ray structures of myosin motor domain mutants G680A in the absence and presence of nucleotide as well as the apo structure of mutant G680V. Our results show that the Gly-680 mutations lead to uncoupling of the reactive thiol region from the surrounding structural elements. Structural and functional data indicate that the mutations induce the preferential population of a state that resembles the ADP-bound state. Moreover, the Gly-680 mutants display greatly reduced dynamic properties, which appear to be related to the recovery of myosin motor function at elevated temperatures.

Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V.,Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ J Biol Chem. 2011 Oct 7;286(40):35051-60. Epub 2011 Aug 13. PMID:21841195^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ. Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V. J Biol Chem. 2011 Oct 7;286(40):35051-60. Epub 2011 Aug 13. PMID:21841195 doi:10.1074/jbc.M111.265298

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2y8i"

@@ Line 1: / Line 1: @@
 ==Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2==
-<StructureSection load='2y8i' size='340' side='right' caption='[[2y8i]], [[Resolution|resolution]] 3.13&Aring;' scene=''>
+<StructureSection load='2y8i' size='340' side='right'caption='[[2y8i]], [[Resolution|resolution]] 3.13&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2y8i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y8I FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2y8i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicdi Dicdi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y8I FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mne|1mne]], [[1d1c|1d1c]], [[1w9l|1w9l]], [[1vom|1vom]], [[1mmn|1mmn]], [[2aka|2aka]], [[2jhr|2jhr]], [[1w9j|1w9j]], [[2x9h|2x9h]], [[1fmv|1fmv]], [[1mma|1mma]], [[2xo8|2xo8]], [[1mmg|1mmg]], [[1w9i|1w9i]], [[1w9k|1w9k]], [[1mnd|1mnd]], [[1jx2|1jx2]], [[1q5g|1q5g]], [[1d0y|1d0y]], [[2xel|2xel]], [[2jj9|2jj9]], [[1lvk|1lvk]], [[1jwy|1jwy]], [[1yv3|1yv3]], [[1fmw|1fmw]], [[1d0x|1d0x]], [[1d1b|1d1b]], [[1g8x|1g8x]], [[1d1a|1d1a]], [[1d0z|1d0z]], [[1mmd|1mmd]], [[2y0r|2y0r]], [[2y9e|2y9e]]</td></tr>
@@ Line 27: / Line 27: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 11735]]
+[[Category: Dicdi]]
+[[Category: Large Structures]]
 [[Category: Myosin ATPase]]
 [[Category: Adamek, N]]

2y8i

From Proteopedia

Revision as of 06:35, 31 July 2019

Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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