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| | ==Crystal structure of ExoY, a unique nucleotidyl cyclase toxin from Pseudomonas aeruginosa== | | ==Crystal structure of ExoY, a unique nucleotidyl cyclase toxin from Pseudomonas aeruginosa== |
| - | <StructureSection load='5xnw' size='340' side='right' caption='[[5xnw]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='5xnw' size='340' side='right'caption='[[5xnw]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5xnw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XNW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XNW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5xnw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XNW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XNW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.201Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">exoY, PA2191 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xnw OCA], [http://pdbe.org/5xnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xnw RCSB], [http://www.ebi.ac.uk/pdbsum/5xnw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xnw ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xnw OCA], [https://pdbe.org/5xnw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xnw RCSB], [https://www.ebi.ac.uk/pdbsum/5xnw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xnw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9I1S4_PSEAE Q9I1S4_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5xnw" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5xnw" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[3D Adenylyl cyclase 3D structures|3D Adenylyl cyclase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Datta, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Khanppnavar, B]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Lyase]] | + | [[Category: Datta S]] |
| - | [[Category: Nucleotidyl cyclase]] | + | [[Category: Khanppnavar B]] |
| - | [[Category: Secondary messenger]]
| + | |
| - | [[Category: Virulence effector]]
| + | |
| Structural highlights
Function
Q9I1S4_PSEAE
Publication Abstract from PubMed
BACKGROUND: The nucleotidyl cyclase toxin ExoY is an important virulence determinant of Pseudomonas aeruginosa that causes severe acute and chronic infections in immune-compromised individuals. Additionally, this unique T3SS effector shows a striking preference for cUMP, a newly identified non-canonical secondary messenger. Thereby, ExoY is also considered as a potential tool to study unexplored cUMP signaling pathways. METHODS: The crystal structure of ExoY was determined at 2.2A resolutions by in-situ proteolysis assisted crystallization and Rosetta-molecular replacement method. Additionally, isothermal calorimetric (ITC) and molecular dynamic (MD) simulation studies were also carried out to gain molecular insights into its substrate specificity and catalysis. RESULTS AND CONCLUSION: ExoY is a partially unfolded protein with higher propensity to form soluble higher-order oligomers. However, with meticulous attempts of removing of disordered regions by proteases, the recalcitrant ExoY could be successfully crystallized. The crystal structure of ExoY revealed similar overall structural fold present in other anthrax toxA family of nucleotidyl cyclases, with two-to-three distinctly conserved regions conferring specificity to eukaryotic binding partner. The in-vitro catalytic preference of ExoY is in the following order: cGMP > cUMP > cAMP > cCMP. The substrate specificity of ExoY mainly depends on its ability to bind NTP in proper geometrical orientations. ExoY also seems to prefer one-metal-ion dependent catalysis than two-metal-ion dependent catalysis. GENERAL SIGNIFICANCE: Our results provide much needed structural insight on ExoY, an important virulence determinant of Pseudomonas aeruginosa and an exciting tool to study non-canonical cNMP signaling pathways. ACCESSION NUMBERS: The structure factors and coordinate files have been deposited in the Protein Data Bank with accession number 5XNW.
Crystal structure and substrate specificity of ExoY, a unique T3SS mediated secreted nucleotidyl cyclase toxin from Pseudomonas aeruginosa.,Khanppnavar B, Datta S Biochim Biophys Acta. 2018 May 30. pii: S0304-4165(18)30153-3. doi:, 10.1016/j.bbagen.2018.05.021. PMID:29859257[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Khanppnavar B, Datta S. Crystal structure and substrate specificity of ExoY, a unique T3SS mediated secreted nucleotidyl cyclase toxin from Pseudomonas aeruginosa. Biochim Biophys Acta. 2018 May 30. pii: S0304-4165(18)30153-3. doi:, 10.1016/j.bbagen.2018.05.021. PMID:29859257 doi:http://dx.doi.org/10.1016/j.bbagen.2018.05.021
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