2jnf
From Proteopedia
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'''Solution structure of fly troponin C, isoform F1''' | '''Solution structure of fly troponin C, isoform F1''' | ||
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[[Category: Nicola, G F.De.]] | [[Category: Nicola, G F.De.]] | ||
[[Category: Pastore, A.]] | [[Category: Pastore, A.]] | ||
| - | [[Category: | + | [[Category: Ef-hand]] |
| - | [[Category: | + | [[Category: Lethocerus indicus]] |
| - | [[Category: | + | [[Category: Metal binding protein]] |
| - | [[Category: | + | [[Category: Stretch activated muscle contraction]] |
| - | [[Category: | + | [[Category: Troponin c]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:04:18 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:04, 4 May 2008
Solution structure of fly troponin C, isoform F1
Overview
To gain a molecular description of how muscles can be activated by mechanical stretch, we have solved the structure of the calcium-loaded F1 isoform of troponin C (TnC) from Lethocerus and characterized its interactions with troponin I (TnI). We show that the presence of only one calcium cation in the fourth EF hand motif is sufficient to induce an open conformation in the C-terminal lobe of F1 TnC, in contrast with what is observed in vertebrate muscle. This lobe interacts in a calcium-independent way both with the N terminus of TnI and, with lower affinity, with a region of TnI equivalent to the switch and inhibitory peptides of vertebrate muscles. Using both synthetic peptides and recombinant proteins, we show that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain. These findings provide insights into mechanically stimulated muscle contraction.
About this Structure
2JNF is a Single protein structure of sequence from Thermomicrobium roseum. Full crystallographic information is available from OCA.
Reference
The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles., De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A, Structure. 2007 Jul;15(7):813-24. PMID:17637342 Page seeded by OCA on Sun May 4 09:04:18 2008
