2joi
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2joi.jpg|left|200px]] | [[Image:2joi.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2joi", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_2joi| PDB=2joi | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''NMR solution structure of hypothetical protein TA0095 from Thermoplasma acidophilum''' | '''NMR solution structure of hypothetical protein TA0095 from Thermoplasma acidophilum''' | ||
| Line 31: | Line 28: | ||
[[Category: Santoro, J.]] | [[Category: Santoro, J.]] | ||
[[Category: Yee, A.]] | [[Category: Yee, A.]] | ||
| - | [[Category: | + | [[Category: Cog4004 orthologous group]] |
| - | [[Category: | + | [[Category: Protein]] |
| - | [[Category: | + | [[Category: Sgc]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Structural genomics consortium]] |
| - | [[Category: | + | [[Category: Thermoplasma acidophilum]] |
| - | [[Category: | + | [[Category: Unknown function]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:07:02 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:07, 4 May 2008
NMR solution structure of hypothetical protein TA0095 from Thermoplasma acidophilum
Overview
TA0095 is a 96-residue hypothetical protein from Thermoplasma acidophilum that exhibits no sequence similarity to any protein of known structure. Also, TA0095 is a member of the COG4004 orthologous group of unknown function found in Archaea bacteria. We determined its three-dimensional structure by NMR methods. The structure displays an alpha/beta two-layer sandwich architecture formed by three alpha-helices and five beta-strands following the order beta1-alpha1-beta2-beta3-beta4-beta5-alpha2-alpha3. Searches for structural homologs indicate that the TA0095 structure belongs to the TBP-like fold, constituting a novel superfamily characterized by an additional C-terminal helix. The TA0095 structure provides a fold common to the COG4004 proteins that will obviously belong to this new superfamily. Most hydrophobic residues conserved in the COG4004 proteins are buried in the structure determined herein, thus underlying their importance for structure stability. Considering that the TA0095 surface shows a large positively charged patch with a high degree of residue conservation within the COG4004 domain, the biological function of TA0095 and the rest of COG4004 proteins might occur through binding a negatively charged molecule. Like other TBP-like fold proteins, the COG4004 proteins might be DNA-binding proteins. The fact that TA0095 is shown to interact with large DNA fragments is in favor of this hypothesis, although nonspecific DNA binding cannot be ruled out.
About this Structure
2JOI is a Single protein structure of sequence from Thermoplasma acidophilum. Full crystallographic information is available from OCA.
Reference
Solution structure of the hypothetical protein TA0095 from Thermoplasma acidophilum: a novel superfamily with a two-layer sandwich architecture., Leon E, Yee A, Ortiz AR, Santoro J, Rico M, Jimenez MA, Protein Sci. 2007 Oct;16(10):2278-86. Epub 2007 Aug 31. PMID:17766377 Page seeded by OCA on Sun May 4 09:07:02 2008
