2jpn
From Proteopedia
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'''Solution Structure of T4 Bacteriophage Helicase Uvsw.1''' | '''Solution Structure of T4 Bacteriophage Helicase Uvsw.1''' | ||
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[[Category: Sivakolundu, S G.]] | [[Category: Sivakolundu, S G.]] | ||
[[Category: White, S W.]] | [[Category: White, S W.]] | ||
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| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Uvsw]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:09:33 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:09, 4 May 2008
Solution Structure of T4 Bacteriophage Helicase Uvsw.1
Overview
The uvsWXY system is implicated in the replication and repair of the bacteriophage T4 genome. Whereas the roles of the recombinase (UvsX) and the recombination mediator protein (UvsY) are known, the precise role of UvsW is unclear. Sequence analysis identifies UvsW as a member of the monomeric SF2 helicase superfamily that translocates nucleic acid substrates via the action of two RecA-like motor domains. Functional homologies to Escherichia coli RecG and biochemical analyses have shown that UvsW interacts with branched nucleic acid substrates, suggesting roles in recombination and the rescue of stalled replication forks. A sequencing error at the 3'-end of the uvsW gene has revealed a second, short open reading frame that encodes a protein of unknown function called UvsW.1. We have determined the crystal structure of UvsW to 2.7A and the NMR solution structure of UvsW.1. UvsW has a four-domain architecture with structural homology to the eukaryotic SF2 helicase, Rad54. A model of the UvsW-ssDNA complex identifies structural elements and conserved residues that may interact with nucleic acid substrates. The NMR solution structure of UvsW.1 reveals a dynamic four-helix bundle with homology to the structure-specific nucleic acid binding module of RecQ helicases.
About this Structure
2JPN is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4., Kerr ID, Sivakolundu S, Li Z, Buchsbaum JC, Knox LA, Kriwacki R, White SW, J Biol Chem. 2007 Nov 23;282(47):34392-400. Epub 2007 Sep 17. PMID:17878153 Page seeded by OCA on Sun May 4 09:09:33 2008
