2jub
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2jub.jpg|left|200px]] | [[Image:2jub.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2jub", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2jub| PDB=2jub | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''Solution structure of IPI*''' | '''Solution structure of IPI*''' | ||
| Line 30: | Line 27: | ||
[[Category: Weber, D J.]] | [[Category: Weber, D J.]] | ||
[[Category: Wright, N T.]] | [[Category: Wright, N T.]] | ||
| - | [[Category: | + | [[Category: Endonuclease inhibitor]] |
| - | [[Category: | + | [[Category: Ipi*]] |
| - | [[Category: | + | [[Category: Solution]] |
| - | [[Category: | + | [[Category: T4 phage]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:17:55 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:17, 4 May 2008
Solution structure of IPI*
Overview
Phage T4 protects its DNA from the two-gene-encoded gmrS/gmrD (glucose-modified hydroxymethylcytosine restriction endonuclease) CT of pathogenic Escherichia coli, CT596, by injecting several hundred copies of the 76-amino-acid-residue nuclease inhibitor, IPI*, into the infected host. Here, the three-dimensional solution structure of mature IPI* is reported as determined by nuclear magnetic resonance techniques using 1290 experimental nuclear Overhauser effect and dipolar coupling constraints ( approximately 17 constraints per residue). Close examination of this oblate-shaped protein structure reveals a novel fold consisting of two small beta-sheets (beta1: B1 and B2; beta2: B3-B5) flanked at the N- and C-termini by alpha-helices (H1 and H2). Such a fold is very compact in shape and allows ejection of IPI* through the narrow 30-A portal and tail tube apertures of the virion without unfolding. Structural and dynamic measurements identify an exposed hydrophobic knob that is a putative gmrS/gmrD-binding site. A single gene from the uropathogenic E. coli UT189, which codes for a gmrS/gmrD-like UT fusion enzyme (with approximately 90% identity to the heterodimeric CT enzyme), has evolved IPI* inhibitor immunity. Analysis of the gmrS/gmrD restriction endonuclease enzyme family and its IPI* family phage antagonists reveals an evolutionary pathway that has elaborated a surprisingly diverse and specifically fitted set of coevolving attack and defense structures.
About this Structure
2JUB is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Restriction endonuclease inhibitor IPI* of bacteriophage T4: a novel structure for a dedicated target., Rifat D, Wright NT, Varney KM, Weber DJ, Black LW, J Mol Biol. 2008 Jan 18;375(3):720-34. Epub 2007 Nov 1. PMID:18037438 Page seeded by OCA on Sun May 4 09:17:55 2008
