2lef
From Proteopedia
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[[Image:2lef.jpg|left|200px]] | [[Image:2lef.jpg|left|200px]] | ||
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| - | + | {{STRUCTURE_2lef| PDB=2lef | SCENE= }} | |
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'''LEF1 HMG DOMAIN (FROM MOUSE), COMPLEXED WITH DNA (15BP), NMR, 12 STRUCTURES''' | '''LEF1 HMG DOMAIN (FROM MOUSE), COMPLEXED WITH DNA (15BP), NMR, 12 STRUCTURES''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2LEF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry | + | 2LEF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1lef 1lef]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Love, J J.]] | [[Category: Love, J J.]] | ||
[[Category: Wright, P E.]] | [[Category: Wright, P E.]] | ||
| - | [[Category: | + | [[Category: Dna bending]] |
| - | + | [[Category: Dna binding]] | |
| - | [[Category: | + | [[Category: Hmg]] |
| - | [[Category: | + | [[Category: Lef1]] |
| - | [[Category: | + | [[Category: Tcr-a]] |
| - | [[Category: | + | [[Category: Transcription factor]] |
| - | [[Category: | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:30:03 2008'' |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:30, 4 May 2008
LEF1 HMG DOMAIN (FROM MOUSE), COMPLEXED WITH DNA (15BP), NMR, 12 STRUCTURES
Overview
Lymphoid enhancer-binding factor (LEF-1) and the closely related T-cell factor 1 (TCF-1) are sequence-specific and cell-type-specific DNA-binding proteins that play important regulatory roles in organogenesis and thymocyte differentiation. LEF-1 participates in regulation of the enhancer associated with the T cell receptor (TCR)-alpha gene by inducing a sharp bend in the DNA and facilitating interactions between Ets-1, PEBP2-alpha, and ATF/CREB, transcription factors bound at sites flanking the LEF-1 site. It seems that LEF-1 plays an architectural role in the assembly and function of this regulatory nucleoprotein complex. LEF-1 recognizes a specific nucleotide sequence through a high-mobility-group (HMG) domain. Proteins containing HMG domains bind DNA in the minor groove, bend the double helix, and recognize four-way junctions and other irregular DNA structures. Here we report the solution structure of a complex of the LEF-1 HMG domain and adjacent basic region with its cognate DNA. The structure reveals the HMG domain bound in the widened minor groove of a markedly distorted and bent double helix. The basic region binds across the narrowed major groove and contributes to DNA recognition.
About this Structure
2LEF is a Single protein structure of sequence from Mus musculus. This structure supersedes the now removed PDB entry 1lef. Full crystallographic information is available from OCA.
Reference
Structural basis for DNA bending by the architectural transcription factor LEF-1., Love JJ, Li X, Case DA, Giese K, Grosschedl R, Wright PE, Nature. 1995 Aug 31;376(6543):791-5. PMID:7651541 Page seeded by OCA on Sun May 4 09:30:03 2008
Categories: Mus musculus | Single protein | Case, D A. | Li, X. | Love, J J. | Wright, P E. | Dna bending | Dna binding | Hmg | Lef1 | Tcr-a | Transcription factor
