2mas

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[[Image:2mas.gif|left|200px]]
[[Image:2mas.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2mas |SIZE=350|CAPTION= <scene name='initialview01'>2mas</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_2mas", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=S1:Description+Not+Provided'>S1</scene>, <scene name='pdbsite=S2:Description+Not+Provided'>S2</scene>, <scene name='pdbsite=S3:Description+Not+Provided'>S3</scene> and <scene name='pdbsite=S4:Description+Not+Provided'>S4</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PIR:2-(4-AMINO-PHENYL)-5-HYDROXYMETHYL-PYRROLIDINE-3,4-DIOL'>PIR</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine_nucleosidase Purine nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= IU-NH FROM C. FASCICULATA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5656 Crithidia fasciculata])
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-->
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|DOMAIN=
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{{STRUCTURE_2mas| PDB=2mas | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mas OCA], [http://www.ebi.ac.uk/pdbsum/2mas PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2mas RCSB]</span>
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}}
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'''PURINE NUCLEOSIDE HYDROLASE WITH A TRANSITION STATE INHIBITOR'''
'''PURINE NUCLEOSIDE HYDROLASE WITH A TRANSITION STATE INHIBITOR'''
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[[Category: Sacchettini, J C.]]
[[Category: Sacchettini, J C.]]
[[Category: Schramm, V L.]]
[[Category: Schramm, V L.]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: inosine]]
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[[Category: Inosine]]
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[[Category: iu-nh]]
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[[Category: Iu-nh]]
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[[Category: purine nucleosidase]]
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[[Category: Purine nucleosidase]]
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[[Category: purine nucleoside hydrolase]]
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[[Category: Purine nucleoside hydrolase]]
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[[Category: uridine]]
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[[Category: Uridine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:31:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:03:47 2008''
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Revision as of 06:31, 4 May 2008

Image:2mas.gif

Template:STRUCTURE 2mas

PURINE NUCLEOSIDE HYDROLASE WITH A TRANSITION STATE INHIBITOR


Overview

Nucleoside N-ribohydrolases are targets for disruption of purine salvage in the protozoan parasites. The structure of a trypanosomal N-ribohydrolase in complex with a transition-state inhibitor is reported at 2.3 A resolution. The nonspecific nucleoside hydrolase from Crithidia fasciculata cocrystallized with p-aminophenyliminoribitol reveals tightly bound Ca2+ as a catalytic site ligand. The complex with the transition-state inhibitor is characterized by (1) large protein conformational changes to create a hydrophobic leaving group site (2) C3'-exo geometry for the inhibitor, typical of a ribooxocarbenium ion (3) stabilization of the ribooxocarbenium analogue between the neighboring group 5'-hydroxyl and bidentate hydrogen bonds to Asn168; and (4) octacoordinate Ca2+ orients a catalytic site water and is liganded to two hydroxyls of the inhibitor. The mechanism is ribooxocarbenium stabilization with weak leaving group activation and is a departure from glucohydrolases which use paired carboxylates to achieve the transition state.

About this Structure

2MAS is a Single protein structure of sequence from Crithidia fasciculata. Full crystallographic information is available from OCA.

Reference

Trypanosomal nucleoside hydrolase. A novel mechanism from the structure with a transition-state inhibitor., Degano M, Almo SC, Sacchettini JC, Schramm VL, Biochemistry. 1998 May 5;37(18):6277-85. PMID:9572842 Page seeded by OCA on Sun May 4 09:31:46 2008

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