2nlw
From Proteopedia
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'''Solution structure of the RRM domain of human eukaryotic initiation factor 3b''' | '''Solution structure of the RRM domain of human eukaryotic initiation factor 3b''' | ||
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[[Category: Lukavsky, P J.]] | [[Category: Lukavsky, P J.]] | ||
[[Category: Tzakos, A G.]] | [[Category: Tzakos, A G.]] | ||
| - | [[Category: | + | [[Category: Eukaryotic initiation factor 3 complex]] |
| - | [[Category: | + | [[Category: Rna recognition motif]] |
| - | [[Category: | + | [[Category: Translation initiation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:37:27 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:37, 4 May 2008
Solution structure of the RRM domain of human eukaryotic initiation factor 3b
Overview
Mammalian eIF3 is a 700-kDa multiprotein complex essential for initiation of protein synthesis in eukaryotic cells. It consists of 13 subunits (eIF3a to -m), among which eIF3b serves as a major scaffolding protein. Here we report the solution structure of the N-terminal RNA recognition motif of human eIF3b (eIF3b-RRM) determined by NMR spectroscopy. The structure reveals a noncanonical RRM with a negatively charged surface in the beta-sheet area contradictory with potential RNA binding activity. Instead, eIF3j, which is required for stable 40 S ribosome binding of the eIF3 complex, specifically binds to the rear alpha-helices of the eIF3b-RRM, opposite to its beta-sheet surface. Moreover, we identify that an N-terminal 69-amino acid peptide of eIF3j is sufficient for binding to eIF3b-RRM and that this interaction is essential for eIF3b-RRM recruitment to the 40 S ribosomal subunit. Our results provide the first structure of an important subdomain of a core eIF3 subunit and detailed insights into protein-protein interactions between two eIF3 subunits required for stable eIF3 recruitment to the 40 S subunit.
About this Structure
2NLW is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit., ElAntak L, Tzakos AG, Locker N, Lukavsky PJ, J Biol Chem. 2007 Mar 16;282(11):8165-74. Epub 2006 Dec 26. PMID:17190833 Page seeded by OCA on Sun May 4 09:37:27 2008
