2np1
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2np1.gif|left|200px]] | [[Image:2np1.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2np1", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_2np1| PDB=2np1 | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''CRYSTAL STRUCTURE OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS''' | '''CRYSTAL STRUCTURE OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS''' | ||
| Line 27: | Line 24: | ||
[[Category: Montfort, W R.]] | [[Category: Montfort, W R.]] | ||
[[Category: Weichsel, A.]] | [[Category: Weichsel, A.]] | ||
| - | [[Category: | + | [[Category: Antihistamine]] |
| - | [[Category: | + | [[Category: Ferric heme]] |
| - | [[Category: | + | [[Category: Lipocalin]] |
| - | [[Category: | + | [[Category: Nitric oxide transport]] |
| - | [[Category: | + | [[Category: Vasodilator]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:43:43 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:43, 4 May 2008
CRYSTAL STRUCTURE OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS
Overview
The nitrophorins are heme-based proteins from the salivary glands of the blood-sucking insect Rhodnius prolixus that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. The nitrophorins also bind tightly to histamine, which is released by the host to induce wound healing. Here we present three crystal structures of nitrophorin 1 (NP1): bound to cyanide, which binds in a manner similar to NO (2.3 A resolution); bound to histamine (2.0 A resolution); and bound to what appears to be NH3 from the crystallization solution (2.0 A resolution). The NP1 structures reveal heme to be sandwiched between strands of a lipocalin-like beta-barrel, and in an arrangement unlike any other gas-transport protein discovered to date. The heme is six-coordinate with a histidine (His 59) on the proximal side, and ligand in a spacious pocket on the distal side. The structures confirm that NO and histamine compete for the same binding pocket and become buried on binding. The dissociation constant for histamine binding was found to be 19 nM, approximately 100-fold lower than that for NO.
About this Structure
2NP1 is a Single protein structure of sequence from Rhodnius prolixus. Full crystallographic information is available from OCA.
Reference
Crystal structures of a nitric oxide transport protein from a blood-sucking insect., Weichsel A, Andersen JF, Champagne DE, Walker FA, Montfort WR, Nat Struct Biol. 1998 Apr;5(4):304-9. PMID:9546222 Page seeded by OCA on Sun May 4 09:43:43 2008
