2i2y

From Proteopedia

(Difference between revisions)

Revision as of 07:26, 24 March 2021

Solution structure of the RRM of SRp20 bound to the RNA CAUC

Structural highlights

2i2y is a 2 chain structure with sequence from Strsg. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	spg and SFRS3 (STRSG)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The sequence-specific RNA-binding proteins SRp20 and 9G8 are the smallest members of the serine- and arginine-rich (SR) protein family, well known for their role in splicing. They also play a role in mRNA export, in particular of histone mRNAs. We present the solution structures of the free 9G8 and SRp20 RNA recognition motifs (RRMs) and of SRp20 RRM in complex with the RNA sequence 5'CAUC3'. The SRp20-RNA structure reveals that although all 4 nt are contacted by the RRM, only the 5' cytosine is primarily recognized in a specific way. This might explain the numerous consensus sequences found by SELEX (systematic evolution of ligands by exponential enrichment) for the RRM of 9G8 and SRp20. Furthermore, we identify a short arginine-rich peptide adjacent to the SRp20 and 9G8 RRMs, which does not contact RNA but is necessary and sufficient for interaction with the export factor Tip-associated protein (TAP). Together, these results provide a molecular description for mRNA and TAP recognition by SRp20 and 9G8.

Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.,Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH EMBO J. 2006 Nov 1;25(21):5126-37. Epub 2006 Oct 12. PMID:17036044^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 2006 Nov 1;25(21):5126-37. Epub 2006 Oct 12. PMID:17036044

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2i2y"

@@ Line 1: / Line 1: @@
 ==Solution structure of the RRM of SRp20 bound to the RNA CAUC==
-<StructureSection load='2i2y' size='340' side='right' caption='[[2i2y]], [[NMR_Ensembles_of_Models | 28 NMR models]]' scene=''>
+<StructureSection load='2i2y' size='340' side='right'caption='[[2i2y]], [[NMR_Ensembles_of_Models | 28 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2i2y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strsg Strsg]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I2Y FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2i2y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Strsg Strsg]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I2Y FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spg and SFRS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 STRSG])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spg and SFRS3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 STRSG])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2y OCA], [http://pdbe.org/2i2y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i2y RCSB], [http://www.ebi.ac.uk/pdbsum/2i2y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i2y ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2y OCA], [https://pdbe.org/2i2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i2y RCSB], [https://www.ebi.ac.uk/pdbsum/2i2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i2y ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 28: / Line 28: @@
 ==See Also==
-*[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]]
+*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
-*[[Srp20-Human Alternative Splicing Factor|Srp20-Human Alternative Splicing Factor]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Strsg]]
 [[Category: Allain, F H]]

2i2y

From Proteopedia

Revision as of 07:26, 24 March 2021

Solution structure of the RRM of SRp20 bound to the RNA CAUC

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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