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2i0w

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Revision as of 07:40, 8 April 2020

Crystal structure analysis of NP24-I, a thaumatin-like protein

Structural highlights

2i0w is a 1 chain structure with sequence from Solanum lycopersicum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NP24_SOLLC] Has antifungal activity against P.betae and F.dahliae. May be involved in disease resistance in tomatoes and/or have a possible role in fruit development and ripening.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of NP24-I, an isoform of the thaumatin-like protein (TLP) NP24 from tomato, has been reported. A prominent acidic cleft is observed between domains I and II of the three-domain structure of this antifungal protein, a feature common to other antifungal TLPs. The defensive role of the TLPs has also been attributed to their beta-1,3-glucanase activity and here too the acidic cleft is reported to play a vital role. NP24 is known to bind beta-glucans and so a linear beta-1,3-glucan molecule has been docked in the interdomain cleft of NP24-I. From the docked complex it is observed that the beta-glucan chain is so positioned in the cleft that a Glu and Asp residue on either side of it may form a catalytic pair to cause the cleavage of a glycosidic bond. NP24 has been reported to be an allergenic protein and an allergenic motif could be identified on the surface of the helical domain II of NP24-I. In addition, some allergenic motifs bearing high similarity/identity with some predicted Ig-E binding motifs of closely related allergenic TLPs like Jun a 3 (Juniperus ashei, from mountain cedar pollen) and banana-TLP have been identified on the molecular surface of NP24-I.

Crystal structure analysis of NP24-I: a thaumatin-like protein.,Ghosh R, Chakrabarti C Planta. 2008 Oct;228(5):883-90. Epub 2008 Jul 24. PMID:18651170^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pressey R. Two isoforms of NP24: a thaumatin-like protein in tomato fruit. Phytochemistry. 1997 Apr;44(7):1241-5. PMID:9115696
↑ Ghosh R, Chakrabarti C. Crystal structure analysis of NP24-I: a thaumatin-like protein. Planta. 2008 Oct;228(5):883-90. Epub 2008 Jul 24. PMID:18651170 doi:10.1007/s00425-008-0790-5

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2i0w"

@@ Line 1: / Line 1: @@
 ==Crystal structure analysis of NP24-I, a thaumatin-like protein==
-<StructureSection load='2i0w' size='340' side='right' caption='[[2i0w]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='2i0w' size='340' side='right'caption='[[2i0w]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2i0w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I0W FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2i0w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I0W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2I0W FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i0w OCA], [http://pdbe.org/2i0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i0w RCSB], [http://www.ebi.ac.uk/pdbsum/2i0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i0w ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2i0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i0w OCA], [http://pdbe.org/2i0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i0w RCSB], [http://www.ebi.ac.uk/pdbsum/2i0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i0w ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Solanum lycopersicum]]
 [[Category: Chakrabarti, C]]

2i0w

From Proteopedia

Revision as of 07:40, 8 April 2020

Crystal structure analysis of NP24-I, a thaumatin-like protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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