2ns5
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2ns5.jpg|left|200px]] | [[Image:2ns5.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2ns5", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2ns5| PDB=2ns5 | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''The conserved N-terminal domain of Par-3 adopts a novel PB1-like structure required for Par-3 oligomerization and apical membrane localization''' | '''The conserved N-terminal domain of Par-3 adopts a novel PB1-like structure required for Par-3 oligomerization and apical membrane localization''' | ||
| Line 29: | Line 26: | ||
[[Category: Wu, H.]] | [[Category: Wu, H.]] | ||
[[Category: Zhang, M.]] | [[Category: Zhang, M.]] | ||
| - | [[Category: | + | [[Category: Asymmetric membrane localization]] |
| - | [[Category: | + | [[Category: Cell polarity]] |
| - | [[Category: | + | [[Category: N-terminal domain]] |
| - | [[Category: | + | [[Category: Par-3]] |
| - | [[Category: | + | [[Category: Pb1 domain]] |
| - | [[Category: | + | [[Category: Signaling protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:50:47 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:50, 4 May 2008
The conserved N-terminal domain of Par-3 adopts a novel PB1-like structure required for Par-3 oligomerization and apical membrane localization
Overview
The evolutionarily conserved Par-3/Par-6/aPKC complex is essential for the establishment and maintenance of polarity of a wide range of cells. Both Par-3 and Par-6 are PDZ domain containing scaffold proteins capable of binding to polarity regulatory proteins. In addition to three PDZ domains, Par-3 also contains a conserved N-terminal oligomerization domain (NTD) that is essential for proper subapical membrane localization and consequently the functions of Par-3. The molecular basis of NTD-mediated Par-3 membrane localization is poorly understood. Here, we describe the structure of a monomeric form of the Par-3 NTD. Unexpectedly, the domain adopts a PB1-like fold with both type-I and type-II structural features. The Par-3 NTD oligomerizes into helical filaments via front-to-back interactions. We further demonstrate that the NTD-mediated membrane localization of Par-3 in MDCK cells is solely attributed to its oligomerization capacity. The data presented in this study suggest that the Par-3 NTD is likely to facilitate the assembly of higher-order Par-3/Par-6/aPKC complex with increased avidities in targeting the complex to the subapical membrane domain and in binding to other polarity-regulating proteins.
About this Structure
2NS5 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and membrane localization., Feng W, Wu H, Chan LN, Zhang M, EMBO J. 2007 Jun 6;26(11):2786-96. Epub 2007 May 3. PMID:17476308 Page seeded by OCA on Sun May 4 09:50:47 2008
