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5ow5

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Revision as of 07:32, 2 September 2020

p60p80-CAMSAP complex

Structural highlights

5ow5 is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Activity:	Microtubule-severing ATPase, with EC number 3.6.4.3
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KTNB1_MOUSE] Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.[HAMAP-Rule:MF_03022] [E9PZI6_MOUSE] Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.[HAMAP-Rule:MF_03023]

Publication Abstract from PubMed

CAMSAP/Patronin family members regulate the organization and stability of microtubule minus ends in various systems ranging from mitotic spindles to differentiated epithelial cells and neurons. Mammalian CAMSAP2 and CAMSAP3 bind to growing microtubule minus ends, where they form stretches of stabilized microtubule lattice. The microtubule-severing ATPase katanin interacts with CAMSAPs and limits the length of CAMSAP-decorated microtubule stretches. Here, by using biochemical, biophysical, and structural approaches, we reveal that a short helical motif conserved in CAMSAP2 and CAMSAP3 binds to the heterodimer formed by the N- and C-terminal domains of katanin subunits p60 and p80, respectively. The identified CAMSAP-katanin binding mode is supported by mutational analysis and genome-editing experiments. It is strikingly similar to the one seen in the ASPM-katanin complex, which is responsible for microtubule minus-end regulation in mitotic spindles. Our work provides a general molecular mechanism for the cooperation of katanin with major microtubule minus-end regulators.

Structural Basis of Formation of the Microtubule Minus-End-Regulating CAMSAP-Katanin Complex.,Jiang K, Faltova L, Hua S, Capitani G, Prota AE, Landgraf C, Volkmer R, Kammerer RA, Steinmetz MO, Akhmanova A Structure. 2018 Mar 6;26(3):375-382.e4. doi: 10.1016/j.str.2017.12.017. Epub 2018, Jan 26. PMID:29395789^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jiang K, Faltova L, Hua S, Capitani G, Prota AE, Landgraf C, Volkmer R, Kammerer RA, Steinmetz MO, Akhmanova A. Structural Basis of Formation of the Microtubule Minus-End-Regulating CAMSAP-Katanin Complex. Structure. 2018 Mar 6;26(3):375-382.e4. doi: 10.1016/j.str.2017.12.017. Epub 2018, Jan 26. PMID:29395789 doi:http://dx.doi.org/10.1016/j.str.2017.12.017

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ow5"

@@ Line 1: / Line 1: @@
 ==p60p80-CAMSAP complex==
-<StructureSection load='5ow5' size='340' side='right' caption='[[5ow5]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+<StructureSection load='5ow5' size='340' side='right'caption='[[5ow5]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ow5]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OW5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OW5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ow5]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OW5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OW5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microtubule-severing_ATPase Microtubule-severing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.3 3.6.4.3] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ow5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ow5 OCA], [http://pdbe.org/5ow5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ow5 RCSB], [http://www.ebi.ac.uk/pdbsum/5ow5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ow5 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ow5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ow5 OCA], [http://pdbe.org/5ow5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ow5 RCSB], [http://www.ebi.ac.uk/pdbsum/5ow5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ow5 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Microtubule-severing ATPase]]
 [[Category: Capitani, G]]

5ow5

From Proteopedia

Revision as of 07:32, 2 September 2020

p60p80-CAMSAP complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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