2nu5
From Proteopedia
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[[Image:2nu5.jpg|left|200px]] | [[Image:2nu5.jpg|left|200px]] | ||
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'''Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine''' | '''Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine''' | ||
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[[Category: Wlodawer, A.]] | [[Category: Wlodawer, A.]] | ||
[[Category: Ziolkowska, N E.]] | [[Category: Ziolkowska, N E.]] | ||
| - | [[Category: | + | [[Category: Antiviral protein,sugar binding protein]] |
| - | [[Category: | + | [[Category: Domain swapping]] |
| - | [[Category: | + | [[Category: Griffithsin]] |
| - | [[Category: | + | [[Category: Hiv]] |
| - | [[Category: | + | [[Category: Lectin]] |
| - | [[Category: | + | [[Category: Mannose binding]] |
| - | [[Category: | + | [[Category: Sar]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:54:56 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:54, 4 May 2008
Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine
Overview
Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes.
About this Structure
2NU5 is a Single protein structure of sequence from Griffithsia sp. q66d336. Full crystallographic information is available from OCA.
Reference
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736 Page seeded by OCA on Sun May 4 09:54:56 2008
