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| | ==QTRT2, the non-catalytic subunit of murine tRNA-Guanine Transglycosylase== | | ==QTRT2, the non-catalytic subunit of murine tRNA-Guanine Transglycosylase== |
| - | <StructureSection load='6fv5' size='340' side='right' caption='[[6fv5]], [[Resolution|resolution]] 2.18Å' scene=''> | + | <StructureSection load='6fv5' size='340' side='right'caption='[[6fv5]], [[Resolution|resolution]] 2.18Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6fv5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FV5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FV5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6fv5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FV5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FV5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.179Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Qtrt2, Qtrtd1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fv5 OCA], [http://pdbe.org/6fv5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fv5 RCSB], [http://www.ebi.ac.uk/pdbsum/6fv5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fv5 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fv5 OCA], [https://pdbe.org/6fv5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fv5 RCSB], [https://www.ebi.ac.uk/pdbsum/6fv5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fv5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/QTRT2_MOUSE QTRT2_MOUSE]] Non-catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_03043]<ref>PMID:19414587</ref> | + | [https://www.uniprot.org/uniprot/QTRT2_MOUSE QTRT2_MOUSE] Non-catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_03043]<ref>PMID:19414587</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6fv5" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6fv5" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[TRNA-guanine transglycosylase 3D structures|TRNA-guanine transglycosylase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Behrens, C]] | + | [[Category: Mus musculus]] |
| - | [[Category: Heine, A]] | + | [[Category: Behrens C]] |
| - | [[Category: Reuter, K]] | + | [[Category: Heine A]] |
| - | [[Category: Tim barrel]] | + | [[Category: Reuter K]] |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Transglycosylase]]
| + | |
| Structural highlights
Function
QTRT2_MOUSE Non-catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_03043][1]
Publication Abstract from PubMed
The bacterial enzyme tRNA-guanine transglycosylase (TGT) is involved in the biosynthesis of queuosine, a modified nucleoside present in the anticodon wobble position of tRNAsHis,Tyr,Asp,Asn. Although it forms a stable homodimer endowed with two active sites, it is, for steric reasons, able to bind and convert only one tRNA molecule at a time. In contrast, its mammalian counterpart constitutes a heterodimer consisting of a catalytic and a noncatalytic subunit, referred to as QTRT1 and QTRT2, respectively. Both subunits are homologous to the bacterial enzyme, yet only QTRT1 possesses all the residues required for substrate binding and catalysis. In mice, genetic inactivation of the TGT results in the uncontrolled oxidation of tetrahydrobiopterin and, accordingly, to phenylketonuria-like symptoms. Due to this fact and the recent finding that mammalian TGT may be utilised for the treatment of multiple sclerosis, this enzyme is of potential medical relevance rendering detailed knowledge about its biochemistry and structural architecture highly desirable. In the present study, we performed the kinetic characterisation of the murine enzyme, investigated potential quaternary structures of QTRT1 and QTRT2 via noncovalent mass spectrometry, and, finally, determined the crystal structure of the murine noncatalytic TGT subunit, QTRT2. In the crystal, QTRT2 is clearly present as a homodimer with striking similarity to that formed by bacterial TGT. In particular, a cluster of four aromatic residues within the interface of the bacterial TGT, which constitutes a "hot spot" for dimer stability, is present in similar constellation in QTRT2.
Homodimer architecture of QTRT2, the noncatalytic subunit of the eukaryotic tRNA-guanine transglycosylase.,Behrens C, Biela I, Petiot-Becard S, Botzanowski T, Cianferani S, Sager CP, Klebe G, Heine A, Reuter K Biochemistry. 2018 Jun 4. doi: 10.1021/acs.biochem.8b00294. PMID:29862811[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Boland C, Hayes P, Santa-Maria I, Nishimura S, Kelly VP. Queuosine formation in eukaryotic tRNA occurs via a mitochondria-localized heteromeric transglycosylase. J Biol Chem. 2009 Jul 3;284(27):18218-27. doi: 10.1074/jbc.M109.002477. Epub 2009, May 4. PMID:19414587 doi:http://dx.doi.org/10.1074/jbc.M109.002477
- ↑ Behrens C, Biela I, Petiot-Becard S, Botzanowski T, Cianferani S, Sager CP, Klebe G, Heine A, Reuter K. Homodimer architecture of QTRT2, the noncatalytic subunit of the eukaryotic tRNA-guanine transglycosylase. Biochemistry. 2018 Jun 4. doi: 10.1021/acs.biochem.8b00294. PMID:29862811 doi:http://dx.doi.org/10.1021/acs.biochem.8b00294
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