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| | ==Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)== | | ==Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)== |
| - | <StructureSection load='2jm3' size='340' side='right' caption='[[2jm3]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2jm3' size='340' side='right'caption='[[2jm3]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jm3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JM3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JM3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jm3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caeel Caeel]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JM3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JM3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">F49E10.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">F49E10.5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jm3 OCA], [http://pdbe.org/2jm3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jm3 RCSB], [http://www.ebi.ac.uk/pdbsum/2jm3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jm3 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jm3 OCA], [https://pdbe.org/2jm3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jm3 RCSB], [https://www.ebi.ac.uk/pdbsum/2jm3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jm3 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CTBP1_CAEEL CTBP1_CAEEL]] Binds DNA and represses gene expression. Plays a role in regulation of life span, possibly by regulating transcription of genes important for lipid metabolism.<ref>PMID:18005989</ref> <ref>PMID:19164523</ref> | + | [[https://www.uniprot.org/uniprot/CTBP1_CAEEL CTBP1_CAEEL]] Binds DNA and represses gene expression. Plays a role in regulation of life span, possibly by regulating transcription of genes important for lipid metabolism.<ref>PMID:18005989</ref> <ref>PMID:19164523</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Caeel]] | | [[Category: Caeel]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Crossley, M]] | | [[Category: Crossley, M]] |
| | [[Category: Liew, C K]] | | [[Category: Liew, C K]] |
| Structural highlights
Function
[CTBP1_CAEEL] Binds DNA and represses gene expression. Plays a role in regulation of life span, possibly by regulating transcription of genes important for lipid metabolism.[1] [2]
Publication Abstract from PubMed
The THAP (Thanatos-associated protein) domain is a recently discovered zinc-binding domain found in proteins involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. It contains a single zinc atom ligated by cysteine and histidine residues within a Cys-X(2-4)-Cys-X(35-53)-Cys-X(2)-His consensus. We have determined the NMR solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP) and show that it adopts a fold containing a treble clef motif, bearing similarity to the zinc finger-associated domain (ZAD) from Drosophila Grauzone. The CtBP THAP domain contains a large, positively charged surface patch and we demonstrate that this domain can bind to double-stranded DNA in an electrophoretic mobility-shift assay. These data, together with existing reports, indicate that THAP domains might exhibit a functional diversity similar to that observed for classical and GATA-type zinc fingers.
Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP).,Liew CK, Crossley M, Mackay JP, Nicholas HR J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nicholas HR, Lowry JA, Wu T, Crossley M. The Caenorhabditis elegans protein CTBP-1 defines a new group of THAP domain-containing CtBP corepressors. J Mol Biol. 2008 Jan 4;375(1):1-11. Epub 2007 Oct 22. PMID:18005989 doi:http://dx.doi.org/10.1016/j.jmb.2007.10.041
- ↑ Chen S, Whetstine JR, Ghosh S, Hanover JA, Gali RR, Grosu P, Shi Y. The conserved NAD(H)-dependent corepressor CTBP-1 regulates Caenorhabditis elegans life span. Proc Natl Acad Sci U S A. 2009 Feb 3;106(5):1496-501. doi:, 10.1073/pnas.0802674106. Epub 2009 Jan 21. PMID:19164523 doi:http://dx.doi.org/10.1073/pnas.0802674106
- ↑ Liew CK, Crossley M, Mackay JP, Nicholas HR. Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP). J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978 doi:S0022-2836(06)01598-1
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