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2nx9

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[[Image:2nx9.jpg|left|200px]]
[[Image:2nx9.jpg|left|200px]]
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{{Structure
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|PDB= 2nx9 |SIZE=350|CAPTION= <scene name='initialview01'>2nx9</scene>, resolution 1.70&Aring;
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The line below this paragraph, containing "STRUCTURE_2nx9", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxaloacetate_decarboxylase Oxaloacetate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.3 4.1.1.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= oadA-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
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|DOMAIN=
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{{STRUCTURE_2nx9| PDB=2nx9 | SCENE= }}
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|RELATEDENTRY=[[1rqh|1RQH]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nx9 OCA], [http://www.ebi.ac.uk/pdbsum/2nx9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nx9 RCSB]</span>
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}}
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'''Crystal structure of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump from Vibrio cholerae'''
'''Crystal structure of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump from Vibrio cholerae'''
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[[Category: Dimroth, P.]]
[[Category: Dimroth, P.]]
[[Category: Studer, R.]]
[[Category: Studer, R.]]
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[[Category: biotin enzyme]]
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[[Category: Biotin enzyme]]
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[[Category: carboxyltransferase structure]]
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[[Category: Carboxyltransferase structure]]
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[[Category: oxaloacetate decarboxylase]]
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[[Category: Oxaloacetate decarboxylase]]
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[[Category: tim-barrel fold]]
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[[Category: Tim-barrel fold]]
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[[Category: zn2+ binding site]]
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[[Category: Zn2+ binding site]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:01:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:09:45 2008''
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Revision as of 07:01, 4 May 2008

Image:2nx9.jpg

Template:STRUCTURE 2nx9

Crystal structure of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump from Vibrio cholerae


Overview

Oxaloacetate decarboxylase is a membrane-bound multiprotein complex that couples oxaloacetate decarboxylation to sodium ion transport across the membrane. The initial reaction catalyzed by this enzyme machinery is the carboxyl transfer from oxaloacetate to the prosthetic biotin group. The crystal structure of the carboxyltransferase at 1.7 A resolution shows a dimer of alpha(8)beta(8) barrels with an active site metal ion, identified spectroscopically as Zn(2+), at the bottom of a deep cleft. The enzyme is completely inactivated by specific mutagenesis of Asp17, His207 and His209, which serve as ligands for the Zn(2+) metal ion, or by Lys178 near the active site, suggesting that Zn(2+) as well as Lys178 are essential for the catalysis. In the present structure this lysine residue is hydrogen-bonded to Cys148. A potential role of Lys178 as initial acceptor of the carboxyl group from oxaloacetate is discussed.

About this Structure

2NX9 is a Single protein structure of sequence from Vibrio cholerae. Full crystallographic information is available from OCA.

Reference

Crystal structure of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump from Vibrio cholerae., Studer R, Dahinden P, Wang WW, Auchli Y, Li XD, Dimroth P, J Mol Biol. 2007 Mar 23;367(2):547-57. Epub 2006 Dec 19. PMID:17270211 Page seeded by OCA on Sun May 4 10:01:44 2008

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