2jni

From Proteopedia

(Difference between revisions)

Revision as of 07:56, 15 April 2020

Spatial structure of antimicrobial peptide arenicin-2 in aqueous solution

Structural highlights

2jni is a 1 chain structure with sequence from Arema. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ANN2_AREMA] Has antimicrobial activity against the Gram-negative bacteria E.coli and P.mirabilis, the Gram-positive bacterium L.monocytogenes and the yeast C.albicans.^[1] ^[2]

Publication Abstract from PubMed

Arenicins are 21-residue cationic antimicrobial peptides, isolated from marine polychaeta Arenicola marina. In order to determine a high-resolution three-dimensional structure of arenicin-2, the recombinant peptide was overexpressed as a fused form in Escherichia coli. Both arenicin isoforms were synthesized using the Fmoc-based solid-phase strategy. Recombinant and synthetic arenicins were purified, and their antimicrobial and spectroscopic properties were analyzed. NMR investigation shows that in water solution arenicin-2 displays a prolonged beta-hairpin, formed by two antiparallel beta-strands and stabilized by one disulfide and nine hydrogen bonds. A significant right-handed twist in the beta-sheet is deprived the peptide surface of amphipathicity. CD spectroscopic analysis indicates that arenicin-2 binds to the SDS and DPC micelles, and conformation of the peptide is significantly changed upon binding. Arenicin strongly binds to anionic lipid (POPE/POPG) vesicles in contrast with zwitterionic (POPC) ones. These results suggest that arenicins are membrane active peptides and point to possible mechanism of their selectivity toward bacterial cells.

Recombinant expression, synthesis, purification, and solution structure of arenicin.,Ovchinnikova TV, Shenkarev ZO, Nadezhdin KD, Balandin SV, Zhmak MN, Kudelina IA, Finkina EI, Kokryakov VN, Arseniev AS Biochem Biophys Res Commun. 2007 Aug 17;360(1):156-62. Epub 2007 Jun 14. PMID:17585874^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ovchinnikova TV, Aleshina GM, Balandin SV, Krasnosdembskaya AD, Markelov ML, Frolova EI, Leonova YF, Tagaev AA, Krasnodembsky EG, Kokryakov VN. Purification and primary structure of two isoforms of arenicin, a novel antimicrobial peptide from marine polychaeta Arenicola marina. FEBS Lett. 2004 Nov 5;577(1-2):209-14. PMID:15527787 doi:http://dx.doi.org/10.1016/j.febslet.2004.10.012
↑ Andra J, Jakovkin I, Grotzinger J, Hecht O, Krasnosdembskaya AD, Goldmann T, Gutsmann T, Leippe M. Structure and mode of action of the antimicrobial peptide arenicin. Biochem J. 2008 Feb 15;410(1):113-22. PMID:17935487 doi:http://dx.doi.org/BJ20071051
↑ Ovchinnikova TV, Shenkarev ZO, Nadezhdin KD, Balandin SV, Zhmak MN, Kudelina IA, Finkina EI, Kokryakov VN, Arseniev AS. Recombinant expression, synthesis, purification, and solution structure of arenicin. Biochem Biophys Res Commun. 2007 Aug 17;360(1):156-62. Epub 2007 Jun 14. PMID:17585874 doi:http://dx.doi.org/S0006-291X(07)01245-4

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jni"

@@ Line 1: / Line 1: @@
 ==Spatial structure of antimicrobial peptide arenicin-2 in aqueous solution==
-<StructureSection load='2jni' size='340' side='right' caption='[[2jni]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2jni' size='340' side='right'caption='[[2jni]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jni]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arema Arema]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JNI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jni]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arema Arema]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2JNI FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jni OCA], [http://pdbe.org/2jni PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jni RCSB], [http://www.ebi.ac.uk/pdbsum/2jni PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jni ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2jni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jni OCA], [http://pdbe.org/2jni PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jni RCSB], [http://www.ebi.ac.uk/pdbsum/2jni PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jni ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 </StructureSection>
 [[Category: Arema]]
+[[Category: Large Structures]]
 [[Category: Arseniev, A S]]
 [[Category: Balandin, S V]]

2jni

From Proteopedia

Revision as of 07:56, 15 April 2020

Spatial structure of antimicrobial peptide arenicin-2 in aqueous solution

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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