2nyk
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2nyk.jpg|left|200px]] | [[Image:2nyk.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2nyk", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_2nyk| PDB=2nyk | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of m157 from mouse cytomegalovirus''' | '''Crystal structure of m157 from mouse cytomegalovirus''' | ||
| Line 26: | Line 23: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Garcia, K C.]] | [[Category: Garcia, K C.]] | ||
| - | [[Category: | + | [[Category: Ly49]] |
| - | [[Category: | + | [[Category: M157]] |
| - | [[Category: | + | [[Category: Mhc]] |
| - | [[Category: | + | [[Category: Nk cell]] |
| - | [[Category: | + | [[Category: Viral protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:04:57 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:04, 4 May 2008
Crystal structure of m157 from mouse cytomegalovirus
Overview
Natural killer (NK) cells express activating and inhibitory receptors that, in concert, survey cells for proper expression of cell surface major histocompatibility complex (MHC) class I molecules. The mouse cytomegalovirus encodes an MHC-like protein, m157, which is the only known viral antigen to date capable of engaging both activating (Ly49H) and inhibitory (Ly49I) NK cell receptors. We have determined the 3D structure of m157 and studied its biochemical and cellular interactions with the Ly49H and Ly49I receptors. m157 has a characteristic MHC-fold, yet possesses several unique structural features not found in other MHC class I-like molecules. m157 does not bind peptides or other small ligands, nor does it associate with beta(2)-microglobulin. Instead, m157 engages in extensive intra- and intermolecular interactions within and between its domains to generate a compact minimal MHC-like molecule. m157's binding affinity for Ly49I (K(d) approximately 0.2 microM) is significantly higher than that of classical inhibitory Ly49-MHC interactions. Analysis of viral escape mutations on m157 that render it resistant to NK killing reveals that it is likely to be recognized by Ly49H in a binding mode that differs from Ly49/MHC-I. In addition, Ly49H+ NK cells can efficiently lyse RMA cells expressing m157, despite the presence of native MHC class I. Collectively, our results show that m157 represents a structurally divergent form of MHC class I-like proteins that directly engage Ly49 receptors with appreciable affinity in a noncanonical fashion.
About this Structure
2NYK is a Single protein structure of sequence from Murid herpesvirus 1. Full crystallographic information is available from OCA.
Reference
Structural elucidation of the m157 mouse cytomegalovirus ligand for Ly49 natural killer cell receptors., Adams EJ, Juo ZS, Venook RT, Boulanger MJ, Arase H, Lanier LL, Garcia KC, Proc Natl Acad Sci U S A. 2007 Jun 12;104(24):10128-33. Epub 2007 May 30. PMID:17537914 Page seeded by OCA on Sun May 4 10:04:57 2008
Categories: Murid herpesvirus 1 | Single protein | Garcia, K C. | Ly49 | M157 | Mhc | Nk cell | Viral protein
