2o3d
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2o3d.gif|left|200px]] | [[Image:2o3d.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2o3d", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2o3d| PDB=2o3d | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structure of human SF2/ASF RNA recognition motif 2 (RRM2)''' | '''Structure of human SF2/ASF RNA recognition motif 2 (RRM2)''' | ||
| Line 31: | Line 28: | ||
[[Category: Tintaru, A M.]] | [[Category: Tintaru, A M.]] | ||
[[Category: Wilson, S A.]] | [[Category: Wilson, S A.]] | ||
| - | [[Category: | + | [[Category: Rna binding protein]] |
| - | [[Category: | + | [[Category: Rrm domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:16:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:16, 4 May 2008
Structure of human SF2/ASF RNA recognition motif 2 (RRM2)
Overview
The serine/arginine-rich (SR) protein splicing factor 2/alternative splicing factor (SF2/ASF) has a role in splicing, stability, export and translation of messenger RNA. Here, we present the structure of the RNA recognition motif (RRM) 2 from SF2/ASF, which has an RRM fold with a considerably extended loop 5 region, containing a two-stranded beta-sheet. The loop 5 extension places the previously identified SR protein kinase 1 docking sequence largely within the RRM fold. We show that RRM2 binds to RNA in a new way, by using a tryptophan within a conserved SWQLKD motif that resides on helix alpha1, together with amino acids from strand beta2 and a histidine on loop 5. The linker connecting RRM1 and RRM2 contains arginine residues, which provide a binding site for the mRNA export factor TAP, and when TAP binds to this region it displaces RNA bound to RRM2.
About this Structure
2O3D is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural and functional analysis of RNA and TAP binding to SF2/ASF., Tintaru AM, Hautbergue GM, Hounslow AM, Hung ML, Lian LY, Craven CJ, Wilson SA, EMBO Rep. 2007 Aug;8(8):756-62. Epub 2007 Jul 13. PMID:17668007 Page seeded by OCA on Sun May 4 10:16:36 2008
