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| | ==Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikE with the HMA domain of Pikm-1 from rice (Oryza sativa)== | | ==Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikE with the HMA domain of Pikm-1 from rice (Oryza sativa)== |
| - | <StructureSection load='6fub' size='340' side='right' caption='[[6fub]], [[Resolution|resolution]] 1.30Å' scene=''> | + | <StructureSection load='6fub' size='340' side='right'caption='[[6fub]], [[Resolution|resolution]] 1.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6fub]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpi_841383 Bpi 841383] and [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FUB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FUB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6fub]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group] and [https://en.wikipedia.org/wiki/Pyricularia_oryzae Pyricularia oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FUB FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6fu9|6fu9]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AVR-Pik ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=318829 BPI 841383]), Pikm1-TS, Pi-km1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fub OCA], [https://pdbe.org/6fub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fub RCSB], [https://www.ebi.ac.uk/pdbsum/6fub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fub ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fub OCA], [http://pdbe.org/6fub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fub RCSB], [http://www.ebi.ac.uk/pdbsum/6fub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fub ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/C4B8C2_PYROR C4B8C2_PYROR] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpi 841383]] | + | [[Category: Large Structures]] |
| - | [[Category: Japanese rice]] | + | [[Category: Oryza sativa Japonica Group]] |
| - | [[Category: Banfield, M J]] | + | [[Category: Pyricularia oryzae]] |
| - | [[Category: Concepcion, J C.De la]] | + | [[Category: Banfield MJ]] |
| - | [[Category: Franceschetti, M]] | + | [[Category: De la Concepcion JC]] |
| - | [[Category: Antifungal protein]] | + | [[Category: Franceschetti M]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Fungal effector]]
| + | |
| - | [[Category: Plant immunity]]
| + | |
| - | [[Category: Plant nlr]]
| + | |
| Structural highlights
Function
C4B8C2_PYROR
Publication Abstract from PubMed
Accelerated adaptive evolution is a hallmark of plant-pathogen interactions. Plant intracellular immune receptors (NLRs) often occur as allelic series with differential pathogen specificities. The determinants of this specificity remain largely unknown. Here, we unravelled the biophysical and structural basis of expanded specificity in the allelic rice NLR Pik, which responds to the effector AVR-Pik from the rice blast pathogen Magnaporthe oryzae. Rice plants expressing the Pikm allele resist infection by blast strains expressing any of three AVR-Pik effector variants, whereas those expressing Pikp only respond to one. Unlike Pikp, the integrated heavy metal-associated (HMA) domain of Pikm binds with high affinity to each of the three recognized effector variants, and variation at binding interfaces between effectors and Pikp-HMA or Pikm-HMA domains encodes specificity. By understanding how co-evolution has shaped the response profile of an allelic NLR, we highlight how natural selection drove the emergence of new receptor specificities. This work has implications for the engineering of NLRs with improved utility in agriculture.
Polymorphic residues in rice NLRs expand binding and response to effectors of the blast pathogen.,De la Concepcion JC, Franceschetti M, Maqbool A, Saitoh H, Terauchi R, Kamoun S, Banfield MJ Nat Plants. 2018 Jul 9. pii: 10.1038/s41477-018-0194-x. doi:, 10.1038/s41477-018-0194-x. PMID:29988155[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ De la Concepcion JC, Franceschetti M, Maqbool A, Saitoh H, Terauchi R, Kamoun S, Banfield MJ. Polymorphic residues in rice NLRs expand binding and response to effectors of the blast pathogen. Nat Plants. 2018 Jul 9. pii: 10.1038/s41477-018-0194-x. doi:, 10.1038/s41477-018-0194-x. PMID:29988155 doi:http://dx.doi.org/10.1038/s41477-018-0194-x
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