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2jxm

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Revision as of 08:01, 7 April 2021

Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex

Structural highlights

2jxm is a 2 chain structure with sequence from Ccap 1490/1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1b3i, 1ci3
Gene:	petE (CCAP 1490/1), petA (CCAP 1490/1)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PLAS_PROHO] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecular pseudocontact shifts and chemical shift perturbations were used as restraints in docking calculations to determine the structure of the wild-type Pc-cytf complex. The orientation of Pc is similar to orientations found in Pc-cytf complexes from other sources. Electrostatics seems to play a modest role in complex formation. A large variability in the ensemble of lowest energy structures indicates a dynamic nature of the complex. Two unusual hydrophobic patch residues in Pc have been mutated to the residues found in other plastocyanins (Y12G/P14L). The binding constants are similar for the complexes of cytf with wild-type Pc and mutant Pc, but the chemical shift perturbations are smaller for the complex with mutant Pc. Docking calculations for the Y12G/P14L Pc-cytf complex did not produce a converged ensemble of structures. Simulations of the dynamics were performed using the observed averaged NMR parameters as input. The results indicate a surprisingly large amplitude of mobility of Y12G/P14L Pc within the complex. It is concluded that the double mutation shifts the complex further from the well-defined toward the encounter state.

Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica.,Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:18201089^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M. Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica. J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:18201089 doi:10.1021/ja077453p

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jxm"

@@ Line 1: / Line 1: @@
-{{Large structure}}
 ==Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex==
-<StructureSection load='2jxm' size='340' side='right' caption='[[2jxm]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2jxm' size='340' side='right'caption='[[2jxm]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ccap_1490/1 Ccap 1490/1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JXM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jxm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ccap_1490/1 Ccap 1490/1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JXM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1b3i|1b3i]], [[1ci3|1ci3]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1b3i|1b3i]], [[1ci3|1ci3]]</div></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">petE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223 CCAP 1490/1]), petA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223 CCAP 1490/1])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">petE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223 CCAP 1490/1]), petA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223 CCAP 1490/1])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jxm OCA], [http://pdbe.org/2jxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jxm RCSB], [http://www.ebi.ac.uk/pdbsum/2jxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jxm ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jxm OCA], [https://pdbe.org/2jxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jxm RCSB], [https://www.ebi.ac.uk/pdbsum/2jxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jxm ProSAT]</span></td></tr>
 </table>
-{{Large structure}}
 == Function ==
-[[http://www.uniprot.org/uniprot/PLAS_PROHO PLAS_PROHO]] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
+[[https://www.uniprot.org/uniprot/PLAS_PROHO PLAS_PROHO]] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 32: @@
 ==See Also==
-*[[Cytochrome f|Cytochrome f]]
+*[[Cytochrome f 3D structures|Cytochrome f 3D structures]]
 == References ==
 <references/>
@@ Line 39: / Line 38: @@
 </StructureSection>
 [[Category: Ccap 1490/1]]
+[[Category: Large Structures]]
 [[Category: Baranova, M]]
 [[Category: Bullerjahn, G]]

2jxm

From Proteopedia

Revision as of 08:01, 7 April 2021

Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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