2o6n
From Proteopedia
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[[Image:2o6n.jpg|left|200px]] | [[Image:2o6n.jpg|left|200px]] | ||
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| - | + | {{STRUCTURE_2o6n| PDB=2o6n | SCENE= }} | |
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'''RH4B: designed right-handed coiled coil tetramer with all biological amino acids''' | '''RH4B: designed right-handed coiled coil tetramer with all biological amino acids''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2O6N is a [[Single protein]] structure | + | 2O6N is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O6N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Alber, T.]] | [[Category: Alber, T.]] | ||
[[Category: Sales, M.]] | [[Category: Sales, M.]] | ||
| - | [[Category: | + | [[Category: Coiled coil]] |
| - | [[Category: | + | [[Category: De novo protein]] |
| - | [[Category: | + | [[Category: Right-handed]] |
| - | [[Category: | + | [[Category: Tetramer]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:23:35 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:23, 4 May 2008
RH4B: designed right-handed coiled coil tetramer with all biological amino acids
Overview
The previous design of an unprecedented family of two-, three-, and four-helical, right-handed coiled coils utilized nonbiological amino acids to efficiently pack spaces in the oligomer cores. Here we show that a stable, right-handed parallel tetrameric coiled coil, called RH4B, can be designed entirely using biological amino acids. The X-ray crystal structure of RH4B was determined to 1.1 Angstrom resolution using a designed metal binding site to coordinate a single Yb(2+) ion per 33-amino acid polypeptide chain. The resulting experimental phases were particularly accurate, and the experimental electron density map provided an especially clear, unbiased view of the molecule. The RH4B structure closely matched the design, with equivalent core rotamers and an overall root-mean-square deviation for the N-terminal repeat of the tetramer of 0.24 Angstrom. The clarity and resolution of the electron density map, however, revealed alternate rotamers and structural differences between the three sequence repeats in the molecule. These results suggest that the RH4B structure populates an unanticipated variety of structures.
About this Structure
2O6N is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Structure of a designed, right-handed coiled-coil tetramer containing all biological amino acids., Sales M, Plecs JJ, Holton JM, Alber T, Protein Sci. 2007 Oct;16(10):2224-32. Epub 2007 Aug 31. PMID:17766380 Page seeded by OCA on Sun May 4 10:23:35 2008
