2o8a

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[[Image:2o8a.jpg|left|200px]]
[[Image:2o8a.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2o8a |SIZE=350|CAPTION= <scene name='initialview01'>2o8a</scene>, resolution 2.610&Aring;
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The line below this paragraph, containing "STRUCTURE_2o8a", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= Ppp1cc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]), Ppp1r2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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|DOMAIN=
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{{STRUCTURE_2o8a| PDB=2o8a | SCENE= }}
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|RELATEDENTRY=[[2o8g|2O8G]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o8a OCA], [http://www.ebi.ac.uk/pdbsum/2o8a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o8a RCSB]</span>
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}}
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'''rat PP1cgamma complexed with mouse inhibitor-2'''
'''rat PP1cgamma complexed with mouse inhibitor-2'''
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Hurley, T D.]]
[[Category: Hurley, T D.]]
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[[Category: hydrolase/inhibitor complex]]
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[[Category: Hydrolase/inhibitor complex]]
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[[Category: inhibitor-2]]
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[[Category: Inhibitor-2]]
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[[Category: protein phosphatase]]
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[[Category: Protein phosphatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:26:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:14:07 2008''
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Revision as of 07:26, 4 May 2008

Image:2o8a.jpg

Template:STRUCTURE 2o8a

rat PP1cgamma complexed with mouse inhibitor-2


Overview

The functional specificity of type 1 protein phosphatases (PP1) depends on the associated regulatory/targeting and inhibitory subunits. To gain insights into the mechanism of PP1 regulation by inhibitor-2, an ancient and intrinsically disordered regulator, we solved the crystal structure of the complex to 2.5A resolution. Our studies show that, when complexed with PP1c, I-2 acquires three regions of order: site 1, residues 12-17, binds adjacent to a region recognized by many PP1 regulators; site 2, amino acids 44-56, interacts along the RVXF binding groove through an unsuspected sequence, KSQKW; and site 3, residues 130-169, forms alpha-helical regions that lie across the substrate-binding cleft. Specifically, residues 148-151 interact at the catalytic center, displacing essential metal ions, accounting for both rapid inhibition and slower inactivation of PP1c. Thus, our structure provides novel insights into the mechanism of PP1 inhibition and subsequent reactivation, has broad implications for the physiological regulation of PP1, and highlights common inhibitory interactions among phosphoprotein phosphatase family members.

About this Structure

2O8A is a Protein complex structure of sequences from Mus musculus and Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structural basis for regulation of protein phosphatase 1 by inhibitor-2., Hurley TD, Yang J, Zhang L, Goodwin KD, Zou Q, Cortese M, Dunker AK, DePaoli-Roach AA, J Biol Chem. 2007 Sep 28;282(39):28874-83. Epub 2007 Jul 18. PMID:17636256 Page seeded by OCA on Sun May 4 10:26:44 2008

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