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2o8v
From Proteopedia
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[[Image:2o8v.gif|left|200px]] | [[Image:2o8v.gif|left|200px]] | ||
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'''PAPS reductase in a covalent complex with thioredoxin C35A''' | '''PAPS reductase in a covalent complex with thioredoxin C35A''' | ||
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3'-Phosphoadenosine-5'-phosphosulfate reductase in complex with thioredoxin: a structural snapshot in the catalytic cycle., Chartron J, Shiau C, Stout CD, Carroll KS, Biochemistry. 2007 Apr 3;46(13):3942-51. Epub 2007 Mar 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17352498 17352498] | 3'-Phosphoadenosine-5'-phosphosulfate reductase in complex with thioredoxin: a structural snapshot in the catalytic cycle., Chartron J, Shiau C, Stout CD, Carroll KS, Biochemistry. 2007 Apr 3;46(13):3942-51. Epub 2007 Mar 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17352498 17352498] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| - | [[Category: Phosphoadenylyl-sulfate reductase (thioredoxin)]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Carroll, K S.]] | [[Category: Carroll, K S.]] | ||
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[[Category: Shiau, C.]] | [[Category: Shiau, C.]] | ||
[[Category: Stout, C D.]] | [[Category: Stout, C D.]] | ||
| - | [[Category: | + | [[Category: Disulfide crosslinked complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:28:14 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:28, 4 May 2008
PAPS reductase in a covalent complex with thioredoxin C35A
Overview
The crystal structure of Escherichia coli 3'-phosphoadenosine-5'-phosphosulfate (PAPS) reductase in complex with E. coli thioredoxin 1 (Trx1) has been determined to 3.0 A resolution. The two proteins are covalently linked via a mixed disulfide that forms during nucleophilic attack of Trx's N-terminal cysteine on the Sgamma atom of the PAPS reductase S-sulfocysteine (E-Cys-Sgamma-SO3-), a central intermediate in the catalytic cycle. For the first time in a crystal structure, residues 235-244 in the PAPS reductase C-terminus are observed, depicting an array of interprotein salt bridges between Trx and the strictly conserved glutathione-like sequence, Glu238Cys239Gly240Leu241His242. The structure also reveals a Trx-binding surface adjacent to the active site cleft and regions of PAPS reductase associated with conformational change. Interaction at this site strategically positions Trx to bind the S-sulfated C-terminus and addresses the mechanism for requisite structural rearrangement of this domain. An apparent sulfite-binding pocket at the protein-protein interface explicitly orients the S-sulfocysteine Sgamma atom for nucleophilic attack in a subsequent step. Taken together, the structure of PAPS reductase in complex with Trx highlights the large structural rearrangement required to accomplish sulfonucleotide reduction and suggests a role for Trx in catalysis beyond the paradigm of disulfide reduction.
About this Structure
2O8V is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
3'-Phosphoadenosine-5'-phosphosulfate reductase in complex with thioredoxin: a structural snapshot in the catalytic cycle., Chartron J, Shiau C, Stout CD, Carroll KS, Biochemistry. 2007 Apr 3;46(13):3942-51. Epub 2007 Mar 13. PMID:17352498 Page seeded by OCA on Sun May 4 10:28:14 2008
