6h02

From Proteopedia

(Difference between revisions)

Revision as of 07:34, 29 August 2018

Crystal structure of human Mediator subunit MED23

Structural highlights

6h02 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[MED23_HUMAN] Autosomal recessive non-syndromic intellectual disability. The disease is caused by mutations affecting the gene represented in this entry.

Function

[MED23_HUMAN] Required for transcriptional activation subsequent to the assembly of the pre-initiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Sur2 is a metazoan Mediator subunit that interacts with the adenovirus E1A protein and functions in a mitogen-activated protein kinase pathway required for vulva development in Caenorhabditis elegans. We generated sur2-/- embryonic stem cells to analyze its function as a mammalian Mediator component. Our results show that Sur2 forms a subcomplex of the Mediator with two other subunits, TRAP/Med100 and 95. Knock-out of Sur2 prevents activation by E1A-CR3 and the mitogen-activated protein kinase-regulated ETS transcription factor Elk-1, but not by multiple other transcription factors. These results imply that specific activation domains stimulate transcription by binding to distinct Mediator subunits. Activation by E1A and Elk-1 requires recruitment of Mediator to a promoter by binding to its Sur2 subunit.

Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit.,Stevens JL, Cantin GT, Wang G, Shevchenko A, Shevchenko A, Berk AJ Science. 2002 Apr 26;296(5568):755-8. Epub 2002 Apr 4. PMID:11934987^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Boyer TG, Martin ME, Lees E, Ricciardi RP, Berk AJ. Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein. Nature. 1999 May 20;399(6733):276-9. PMID:10353252 doi:http://dx.doi.org/10.1038/20466
↑ Mo X, Kowenz-Leutz E, Xu H, Leutz A. Ras induces mediator complex exchange on C/EBP beta. Mol Cell. 2004 Jan 30;13(2):241-50. PMID:14759369
↑ Baek HJ, Kang YK, Roeder RG. Human Mediator enhances basal transcription by facilitating recruitment of transcription factor IIB during preinitiation complex assembly. J Biol Chem. 2006 Jun 2;281(22):15172-81. Epub 2006 Apr 4. PMID:16595664 doi:M601983200
↑ Stevens JL, Cantin GT, Wang G, Shevchenko A, Shevchenko A, Berk AJ. Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit. Science. 2002 Apr 26;296(5568):755-8. Epub 2002 Apr 4. PMID:11934987 doi:http://dx.doi.org/10.1126/science.1068943

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6h02"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6h02 is ON HOLD  until Jan 06 2020
+==Crystal structure of human Mediator subunit MED23==
+<StructureSection load='6h02' size='340' side='right' caption='[[6h02]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6h02]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H02 FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h02 OCA], [http://pdbe.org/6h02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h02 RCSB], [http://www.ebi.ac.uk/pdbsum/6h02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h02 ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[[http://www.uniprot.org/uniprot/MED23_HUMAN MED23_HUMAN]] Autosomal recessive non-syndromic intellectual disability. The disease is caused by mutations affecting the gene represented in this entry.
+== Function ==
+[[http://www.uniprot.org/uniprot/MED23_HUMAN MED23_HUMAN]] Required for transcriptional activation subsequent to the assembly of the pre-initiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.<ref>PMID:10353252</ref> <ref>PMID:14759369</ref> <ref>PMID:16595664</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Sur2 is a metazoan Mediator subunit that interacts with the adenovirus E1A protein and functions in a mitogen-activated protein kinase pathway required for vulva development in Caenorhabditis elegans. We generated sur2-/- embryonic stem cells to analyze its function as a mammalian Mediator component. Our results show that Sur2 forms a subcomplex of the Mediator with two other subunits, TRAP/Med100 and 95. Knock-out of Sur2 prevents activation by E1A-CR3 and the mitogen-activated protein kinase-regulated ETS transcription factor Elk-1, but not by multiple other transcription factors. These results imply that specific activation domains stimulate transcription by binding to distinct Mediator subunits. Activation by E1A and Elk-1 requires recruitment of Mediator to a promoter by binding to its Sur2 subunit.
-Authors:
+Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit.,Stevens JL, Cantin GT, Wang G, Shevchenko A, Shevchenko A, Berk AJ Science. 2002 Apr 26;296(5568):755-8. Epub 2002 Apr 4. PMID:11934987<ref>PMID:11934987</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6h02" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Clantin, B]]
+[[Category: Monte, D]]
+[[Category: Villeret, V]]
+[[Category: Complex]]
+[[Category: Human]]
+[[Category: Mediator]]
+[[Category: Subunit]]
+[[Category: Transcription]]

6h02

From Proteopedia

Revision as of 07:34, 29 August 2018

Crystal structure of human Mediator subunit MED23

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox