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| | ==Solution structure of murine interleukin 3== | | ==Solution structure of murine interleukin 3== |
| - | <StructureSection load='2l3o' size='340' side='right' caption='[[2l3o]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2l3o' size='340' side='right'caption='[[2l3o]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2l3o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L3O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L3O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2l3o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L3O FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Il3, mCG_13767, mIL-3, RP23-309E16.5-001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Il3, mCG_13767, mIL-3, RP23-309E16.5-001 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3o OCA], [http://pdbe.org/2l3o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l3o RCSB], [http://www.ebi.ac.uk/pdbsum/2l3o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l3o ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3o OCA], [https://pdbe.org/2l3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l3o RCSB], [https://www.ebi.ac.uk/pdbsum/2l3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l3o ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | ==See Also== | | ==See Also== |
| - | *[[Interleukin|Interleukin]] | + | *[[Interleukin 3D structures|Interleukin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Lk3 transgenic mice]] | | [[Category: Lk3 transgenic mice]] |
| | [[Category: Murphy, J M]] | | [[Category: Murphy, J M]] |
| Structural highlights
Publication Abstract from PubMed
Interleukin-3 (IL-3), a cytokine produced primarily by activated T-cells during immune responses, is a crucial regulator of allergic inflammation. The three-dimensional structure of murine IL-3 (mIL-3) has remained elusive owing to its poor solubility and strong tendency toward aggregation under solution conditions typically used for structural studies. Here we describe the solution properties and structure of mIL-3 determined by NMR using an engineered construct of mIL-3 (mIL-3(33-156)). mIL-3 adopts a four-helical bundle fold, typical of proteins belonging to the short-chain cytokine family, and features a core of highly conserved hydrophobic residues. While significant line broadening and peak disappearance were observed in NMR spectra at higher temperatures, there was no evidence for temperature-dependent changes of the oligomeric state of mIL-3(33-156). Further analysis of the temperature dependence of amide (1)H chemical shifts and backbone (15)N relaxation parameters, including (15)N relaxation dispersion, revealed the presence of significant conformational exchange and local conformational heterogeneity. Residues recently shown by mutagenesis to play key roles in beta(IL-3) receptor recognition and activation, which are located within the alpha(A) and alpha(C) helices and aligned on one face of the mIL-3(33-156) structure, are relatively rigid. In contrast, pronounced conformational heterogeneity was observed for a cluster of residues located on the opposite side of mIL-3, which corresponds spatially to sites in the related cytokines human IL-3, IL-5, and GM-CSF that are known to mediate interactions with their respective alpha-receptor subunits. Such conformational heterogeneity may facilitate the interaction of mIL-3 with each of two naturally occurring mIL-3Ralpha isoforms, leading to structurally distinct high-affinity complexes.
Murine interleukin-3: structure, dynamics, and conformational heterogeneity in solution.,Yao S, Young IG, Norton RS, Murphy JM Biochemistry. 2011 Apr 5;50(13):2464-77. Epub 2011 Mar 2. PMID:21329364[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Yao S, Young IG, Norton RS, Murphy JM. Murine interleukin-3: structure, dynamics, and conformational heterogeneity in solution. Biochemistry. 2011 Apr 5;50(13):2464-77. Epub 2011 Mar 2. PMID:21329364 doi:10.1021/bi101810f
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