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| | ==Solution Structure of STAS domain of RV1739c from M. tuberculosis== | | ==Solution Structure of STAS domain of RV1739c from M. tuberculosis== |
| - | <StructureSection load='2kln' size='340' side='right' caption='[[2kln]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''> | + | <StructureSection load='2kln' size='340' side='right'caption='[[2kln]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2kln]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"mycobacterium_tuberculosis_typus_bovinus"_lehmann_and_neumann_1907 "mycobacterium tuberculosis typus bovinus" lehmann and neumann 1907]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KLN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KLN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2kln]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"mycobacterium_tuberculosis_typus_bovinus"_lehmann_and_neumann_1907 "mycobacterium tuberculosis typus bovinus" lehmann and neumann 1907]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KLN FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mb1768c, Rv1739c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1765 "Mycobacterium tuberculosis typus bovinus" Lehmann and Neumann 1907])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mb1768c, Rv1739c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1765 "Mycobacterium tuberculosis typus bovinus" Lehmann and Neumann 1907])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kln OCA], [http://pdbe.org/2kln PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kln RCSB], [http://www.ebi.ac.uk/pdbsum/2kln PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kln ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kln OCA], [https://pdbe.org/2kln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kln RCSB], [https://www.ebi.ac.uk/pdbsum/2kln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kln ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Mycobacterium tuberculosis typus bovinus lehmann and neumann 1907]] | | [[Category: Mycobacterium tuberculosis typus bovinus lehmann and neumann 1907]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Alper, S L]] | | [[Category: Alper, S L]] |
| | [[Category: Rigby, A C]] | | [[Category: Rigby, A C]] |
| Structural highlights
Publication Abstract from PubMed
The structure and intrinsic activities of conserved STAS domains of the ubiquitous SulP/SLC26 anion transporter superfamily have until recently remained unknown. Here we report the heteronuclear, multidimensional NMR spectroscopy solution structure of the STAS domain from the SulP/SLC26 putative anion transporter Rv1739c of Mycobacterium tuberculosis. The 0.87-A root mean square deviation structure revealed a four-stranded beta-sheet with five interspersed alpha-helices, resembling the anti-sigma factor antagonist fold. Rv1739c STAS was shown to be a guanine nucleotide-binding protein, as revealed by nucleotide-dependent quench of intrinsic STAS fluorescence and photoaffinity labeling. NMR chemical shift perturbation analysis partnered with in silico docking calculations identified solvent-exposed STAS residues involved in nucleotide binding. Rv1739c STAS was not an in vitro substrate of mycobacterial kinases or anti-sigma factors. These results demonstrate that Rv1739c STAS binds guanine nucleotides at physiological concentrations and undergoes a ligand-induced conformational change but, unlike anti-sigma factor antagonists, may not mediate signals via phosphorylation.
Solution Structure of the Guanine Nucleotide-binding STAS Domain of SLC26-related SulP Protein Rv1739c from Mycobacterium tuberculosis.,Sharma AK, Ye L, Baer CE, Shanmugasundaram K, Alber T, Alper SL, Rigby AC J Biol Chem. 2011 Mar 11;286(10):8534-44. Epub 2010 Dec 29. PMID:21190940[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sharma AK, Ye L, Baer CE, Shanmugasundaram K, Alber T, Alper SL, Rigby AC. Solution Structure of the Guanine Nucleotide-binding STAS Domain of SLC26-related SulP Protein Rv1739c from Mycobacterium tuberculosis. J Biol Chem. 2011 Mar 11;286(10):8534-44. Epub 2010 Dec 29. PMID:21190940 doi:10.1074/jbc.M110.165449
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