Luciferase
From Proteopedia
(Difference between revisions)
| Line 11: | Line 11: | ||
== Relevance == | == Relevance == | ||
Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>. | Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>. | ||
| + | |||
| + | == 3D Structures of Luciferase == | ||
| + | [[Luciferase 3D structures]] | ||
| + | |||
</StructureSection> | </StructureSection> | ||
| Line 26: | Line 30: | ||
**[[1luc]], [[1brl]] - VhLuc α+β chain<br /> | **[[1luc]], [[1brl]] - VhLuc α+β chain<br /> | ||
**[[1bsl]], [[1xkj]] - VhLuc β chain<br /> | **[[1bsl]], [[1xkj]] - VhLuc β chain<br /> | ||
| + | **[[1vpr]] – Luc residues 866-1241 – ''Lingulodinium polyedrum''<br /> | ||
| + | **[[6aaa]] – Luc - ''Amydetes''<br /> | ||
| + | **[[6aah]], [[6ac3]] – Luc – ''Phrixothrix hirtus''<br /> | ||
| + | **[[6fri]] – Luc β chain – ''Photobacterium leiognathi''<br /> | ||
*Luciferin 2-monooxygenase | *Luciferin 2-monooxygenase | ||
| Line 39: | Line 47: | ||
**[[3ies]], [[3rix]], [[4e5d]] – fLuc+inhibitor<br /> | **[[3ies]], [[3rix]], [[4e5d]] – fLuc+inhibitor<br /> | ||
**[[4g36]] – fLUc + dehydroluciferyl-sulfamoyl adenosine<br /> | **[[4g36]] – fLUc + dehydroluciferyl-sulfamoyl adenosine<br /> | ||
| - | **[[4g37]] – fLUc (mutant) + dehydroluciferyl-sulfamoyl adenosine<br /> | + | **[[4g37]], [[6q2m]] – fLUc (mutant) + dehydroluciferyl-sulfamoyl adenosine<br /> |
**[[5gz2]] – fLuc + thiazole derivative<br /> | **[[5gz2]] – fLuc + thiazole derivative<br /> | ||
**[[5gyz]] – fLuc + AMP + thiazole derivative<br /> | **[[5gyz]] – fLuc + AMP + thiazole derivative<br /> | ||
Revision as of 10:24, 22 October 2019
| |||||||||||
Contents |
3D Structures of Luciferase
Updated on 22-October-2019
Additional information
(2d1s)
Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
- For additional information, see: Colored & Bioluminescent Proteins
References
Additional Literature
- Marques SM, Esteves da Silva JC. Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life. 2009 Jan;61(1):6-17. PMID:18949818 doi:10.1002/iub.134
- Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cell Mol Life Sci. 2010 Feb;67(3):387-404. Epub 2009 Oct 27. PMID:19859663 doi:10.1007/s00018-009-0170-8
External Resources
- Yahoo news coverage of luciferase studies funded research for military applications
- Luciferase at Wikipedia
- Luciferase: June 2006 Molecule of the Month as part of the series of tutorials that are at the RCSB Protein Data Bank and written by David S. Goodsell
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Wayne Decatur, Alexander Berchansky, Loïc Gazquez, Charlene Planchenault, David Canner
