2lme

From Proteopedia

(Difference between revisions)

Revision as of 10:13, 12 May 2021

Solid-state NMR structure of the membrane anchor domain of the trimeric autotransporter YadA

Structural highlights

2lme is a 3 chain structure with sequence from Yere8. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	yadA, YEP0066 (YERE8)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[YADA_YERE8] Collagen-binding outer membrane protein forming a fibrillar matrix on the bacterial cell surface. Promotes initial attachment and invasion of eukaryotic cells. Also protects the bacteria by being responsible for agglutination, serum resistance, complement inactivation and phagocytosis resistance (By similarity).

Publication Abstract from PubMed

Membrane proteins are largely underrepresented among available atomic-resolution structures. The use of detergents in protein purification procedures hinders the formation of well-ordered crystals for X-ray crystallography and leads to slower molecular tumbling, impeding the application of solution-state NMR. Solid-state magic-angle spinning NMR spectroscopy is an emerging method for membrane-protein structural biology that can overcome these technical problems. Here we present the solid-state NMR structure of the transmembrane domain of the Yersinia enterocolitica adhesin A (YadA). The sample was derived from crystallization trials that yielded only poorly diffracting microcrystals. We solved the structure using a single, uniformly (13)C- and (15)N-labeled sample. In addition, solid-state NMR allowed us to acquire information on the flexibility and mobility of parts of the structure, which, in combination with evolutionary conservation information, presents new insights into the autotransport mechanism of YadA.

Membrane-protein structure determination by solid-state NMR spectroscopy of microcrystals.,Shahid SA, Bardiaux B, Franks WT, Krabben L, Habeck M, van Rossum BJ, Linke D Nat Methods. 2012 Dec 7;9(12):1212-7. doi: 10.1038/nmeth.2248. Epub 2012 Nov 11. PMID:23142870^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shahid SA, Bardiaux B, Franks WT, Krabben L, Habeck M, van Rossum BJ, Linke D. Membrane-protein structure determination by solid-state NMR spectroscopy of microcrystals. Nat Methods. 2012 Dec 7;9(12):1212-7. doi: 10.1038/nmeth.2248. Epub 2012 Nov 11. PMID:23142870 doi:http://dx.doi.org/10.1038/nmeth.2248

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lme"

@@ Line 1: / Line 1: @@
-{{Large structure}}
 ==Solid-state NMR structure of the membrane anchor domain of the trimeric autotransporter YadA==
-<StructureSection load='2lme' size='340' side='right' caption='[[2lme]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2lme' size='340' side='right'caption='[[2lme]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2lme]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Yere8 Yere8]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LME FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2lme]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Yere8 Yere8]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LME FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">yadA, YEP0066 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=393305 YERE8])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">yadA, YEP0066 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=393305 YERE8])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lme OCA], [http://pdbe.org/2lme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lme RCSB], [http://www.ebi.ac.uk/pdbsum/2lme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lme ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lme OCA], [https://pdbe.org/2lme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lme RCSB], [https://www.ebi.ac.uk/pdbsum/2lme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lme ProSAT]</span></td></tr>
 </table>
-{{Large structure}}
 == Function ==
-[[http://www.uniprot.org/uniprot/YADA_YERE8 YADA_YERE8]] Collagen-binding outer membrane protein forming a fibrillar matrix on the bacterial cell surface. Promotes initial attachment and invasion of eukaryotic cells. Also protects the bacteria by being responsible for agglutination, serum resistance, complement inactivation and phagocytosis resistance (By similarity).
+[[https://www.uniprot.org/uniprot/YADA_YERE8 YADA_YERE8]] Collagen-binding outer membrane protein forming a fibrillar matrix on the bacterial cell surface. Promotes initial attachment and invasion of eukaryotic cells. Also protects the bacteria by being responsible for agglutination, serum resistance, complement inactivation and phagocytosis resistance (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 20: @@
 ==See Also==
-*[[Adhesin|Adhesin]]
+*[[Adhesin 3D structures|Adhesin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Yere8]]
 [[Category: Bardiaux, B]]

2lme

From Proteopedia

Revision as of 10:13, 12 May 2021

Solid-state NMR structure of the membrane anchor domain of the trimeric autotransporter YadA

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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