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2oqc

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[[Image:2oqc.jpg|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_2oqc", creates the "Structure Box" on the page.
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span>
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|GENE= yxeI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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{{STRUCTURE_2oqc| PDB=2oqc | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqc OCA], [http://www.ebi.ac.uk/pdbsum/2oqc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oqc RCSB]</span>
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'''Crystal Structure of Penicillin V acylase from Bacillus subtilis'''
'''Crystal Structure of Penicillin V acylase from Bacillus subtilis'''
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[[Category: Bacillus subtili]]
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[[Category: choloylglycine hydrolase]]
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[[Category: Choloylglycine hydrolase]]
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[[Category: conjugated bile acid hydrolase]]
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[[Category: Conjugated bile acid hydrolase]]
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[[Category: ntn-hydrolase]]
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[[Category: Ntn-hydrolase]]
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[[Category: penicillin v acylase]]
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[[Category: Penicillin v acylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:21:37 2008''
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Revision as of 08:26, 4 May 2008

Image:2oqc.jpg

Template:STRUCTURE 2oqc

Crystal Structure of Penicillin V acylase from Bacillus subtilis


Overview

Penicillin acylase proteins are amidohydrolase enzymes that cleave penicillins at the amide bond connecting the side chain to their beta-lactam nucleus. An unannotated protein from Bacillus subtilis has been expressed in Escherichia coli, purified and confirmed to possess penicillin V acylase activity. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 4 M sodium formate in 100 mM Tris-HCl buffer pH 8.2. Diffraction data were collected under cryogenic conditions to a spacing of 2.5 A. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 111.0, b = 308.0, c = 56.0 A. The estimated Matthews coefficient was 3.23 A3 Da(-1), corresponding to 62% solvent content. The structure has been solved using molecular-replacement methods with B. sphaericus penicillin V acylase (PDB code 2pva) as the search model.

About this Structure

2OQC is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:16511127 Page seeded by OCA on Sun May 4 11:26:50 2008

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