2oqu

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[[Image:2oqu.jpg|left|200px]]
[[Image:2oqu.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2oqu |SIZE=350|CAPTION= <scene name='initialview01'>2oqu</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_2oqu", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XE:XENON'>XE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2oqu| PDB=2oqu | SCENE= }}
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|RELATEDENTRY=[[2oqn|2OQN]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqu OCA], [http://www.ebi.ac.uk/pdbsum/2oqu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oqu RCSB]</span>
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}}
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'''High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths'''
'''High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths'''
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[[Category: Hao, Q.]]
[[Category: Hao, Q.]]
[[Category: Kim, C U.]]
[[Category: Kim, C U.]]
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[[Category: high pressure cryocooling]]
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[[Category: High pressure cryocooling]]
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[[Category: sad phasing]]
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[[Category: Sad phasing]]
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[[Category: xenon]]
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[[Category: Xenon]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:28:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:21:50 2008''
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Revision as of 08:28, 4 May 2008

Image:2oqu.jpg

Template:STRUCTURE 2oqu

High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths


Overview

Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.

About this Structure

2OQU is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths., Kim CU, Hao Q, Gruner SM, Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:17452791 Page seeded by OCA on Sun May 4 11:28:57 2008

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