2ot3

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[[Image:2ot3.gif|left|200px]]
[[Image:2ot3.gif|left|200px]]
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{{Structure
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|PDB= 2ot3 |SIZE=350|CAPTION= <scene name='initialview01'>2ot3</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_2ot3", creates the "Structure Box" on the page.
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|SITE=
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|GENE= RABGEF1, RABEX5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RAB21, KIAA0118 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_2ot3| PDB=2ot3 | SCENE= }}
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|RELATEDENTRY=[[1txu|1TXU]], [[1z0i|1Z0I]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ot3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ot3 OCA], [http://www.ebi.ac.uk/pdbsum/2ot3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ot3 RCSB]</span>
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'''Crystal structure of rabex-5 VPS9 domain in complex with nucleotide free RAB21'''
'''Crystal structure of rabex-5 VPS9 domain in complex with nucleotide free RAB21'''
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[[Category: Delprato, A.]]
[[Category: Delprato, A.]]
[[Category: Lambright, D.]]
[[Category: Lambright, D.]]
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[[Category: protein transport]]
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[[Category: Protein transport]]
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[[Category: rab21]]
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[[Category: Rab21]]
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[[Category: rabex-5]]
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[[Category: Rabex-5]]
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[[Category: vesicular traffic]]
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[[Category: Vesicular traffic]]
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[[Category: vps9 domain]]
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[[Category: Vps9 domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:36:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:22:45 2008''
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Revision as of 08:36, 4 May 2008

Image:2ot3.gif

Template:STRUCTURE 2ot3

Crystal structure of rabex-5 VPS9 domain in complex with nucleotide free RAB21


Overview

RABEX-5 and other exchange factors with VPS9 domains regulate endocytic trafficking through activation of the Rab family GTPases RAB5, RAB21 and RAB22. Here we report the crystal structure of the RABEX-5 catalytic core in complex with nucleotide-free RAB21, a key intermediate in the exchange reaction pathway. The structure reveals how VPS9 domain exchange factors recognize Rab GTPase substrates, accelerate GDP release and stabilize the nucleotide-free conformation. We further identify an autoinhibitory element in a predicted amphipathic helix located near the C terminus of the VPS9 domain. The autoinhibitory element overlaps with the binding site for the multivalent effector RABAPTIN-5 and potently suppresses the exchange activity of RABEX-5. Autoinhibition can be partially reversed by mutation of conserved residues on the nonpolar face of the predicted amphipathic helix or by assembly of the complex with RABAPTIN-5.

About this Structure

2OT3 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for Rab GTPase activation by VPS9 domain exchange factors., Delprato A, Lambright DG, Nat Struct Mol Biol. 2007 May;14(5):406-12. Epub 2007 Apr 22. PMID:17450153 Page seeded by OCA on Sun May 4 11:36:07 2008

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