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| | ==Structure of the PrgK first periplasmic domain== | | ==Structure of the PrgK first periplasmic domain== |
| - | <StructureSection load='2mky' size='340' side='right' caption='[[2mky]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mky' size='340' side='right'caption='[[2mky]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mky]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium_str._dt2 Salmonella enterica subsp. enterica serovar typhimurium str. dt2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MKY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mky]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium_str._dt2 Salmonella enterica subsp. enterica serovar typhimurium str. dt2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MKY FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prgK, STMDT2_27711 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=568709 Salmonella enterica subsp. enterica serovar Typhimurium str. DT2])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prgK, STMDT2_27711 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=568709 Salmonella enterica subsp. enterica serovar Typhimurium str. DT2])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mky OCA], [http://pdbe.org/2mky PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mky RCSB], [http://www.ebi.ac.uk/pdbsum/2mky PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mky ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mky OCA], [https://pdbe.org/2mky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mky RCSB], [https://www.ebi.ac.uk/pdbsum/2mky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mky ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Salmonella enterica subsp. enterica serovar typhimurium str. dt2]] | | [[Category: Salmonella enterica subsp. enterica serovar typhimurium str. dt2]] |
| | [[Category: Bergeron, J]] | | [[Category: Bergeron, J]] |
| Structural highlights
Publication Abstract from PubMed
The type III secretion system (T3SS) is a large macromolecular assembly found at the surface of many pathogenic Gram-negative bacteria. Its role is to inject toxic "effector" proteins into the cells of infected organisms. The molecular details of the assembly of this large, multimembrane-spanning complex remain poorly understood. Here, we report structural, biochemical, and functional analyses of PrgK, an inner-membrane component of the prototypical Salmonella typhimurium T3SS. We have obtained the atomic structures of the two ring building globular domains and show that the C-terminal transmembrane helix is not essential for assembly and secretion. We also demonstrate that structural rearrangement of the two PrgK globular domains, driven by an interconnecting linker region, may promote oligomerization into ring structures. Finally, we used electron microscopy-guided symmetry modeling to propose a structural model for the intimately associated PrgH-PrgK ring interaction within the assembled basal body.
The Modular Structure of the Inner-Membrane Ring Component PrgK Facilitates Assembly of the Type III Secretion System Basal Body.,Bergeron JR, Worrall LJ, De S, Sgourakis NG, Cheung AH, Lameignere E, Okon M, Wasney GA, Baker D, McIntosh LP, Strynadka NC Structure. 2015 Jan 6;23(1):161-72. doi: 10.1016/j.str.2014.10.021. Epub 2014 Dec, 18. PMID:25533490[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bergeron JR, Worrall LJ, De S, Sgourakis NG, Cheung AH, Lameignere E, Okon M, Wasney GA, Baker D, McIntosh LP, Strynadka NC. The Modular Structure of the Inner-Membrane Ring Component PrgK Facilitates Assembly of the Type III Secretion System Basal Body. Structure. 2015 Jan 6;23(1):161-72. doi: 10.1016/j.str.2014.10.021. Epub 2014 Dec, 18. PMID:25533490 doi:http://dx.doi.org/10.1016/j.str.2014.10.021
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