2oum
From Proteopedia
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'''The first domain of L1 from Thermus thermophilus''' | '''The first domain of L1 from Thermus thermophilus''' | ||
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[[Category: Nikonov, S.]] | [[Category: Nikonov, S.]] | ||
[[Category: Tishchenko, S.]] | [[Category: Tishchenko, S.]] | ||
| - | [[Category: | + | [[Category: Ribosomal protein l1]] |
| - | [[Category: | + | [[Category: Thermus thermophilus]] |
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Revision as of 08:41, 4 May 2008
The first domain of L1 from Thermus thermophilus
Overview
Ribosomal protein L1 has a dual function as a ribosomal protein binding 23S rRNA and as a translational repressor binding its mRNA. L1 is a two-domain protein with N- and C-termini located in domain I. Earlier it was shown that L1 interacts with the same targets on both rRNA and mRNA mainly through domain I. We have suggested that domain I is necessary and sufficient for specific RNA-binding by L1. To test this hypothesis, a truncation mutant of L1 from Thermus thermophilus, representing domain I, was constructed by deletion of the central part of the L1 sequence, which corresponds to domain II. It was shown that the isolated domain I forms stable complexes with specific fragments of both rRNA and mRNA. The crystal structure of the isolated domain I was determined and compared with the structure of this domain within the intact protein L1. This comparison revealed a close similarity of both structures. Our results confirm our suggestion that in protein L1 its domain I alone is sufficient for specific RNA binding, whereas domain II stabilizes the L1-rRNA complex.
About this Structure
2OUM is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Domain I of ribosomal protein L1 is sufficient for specific RNA binding., Tishchenko S, Nikonova E, Kljashtorny V, Kostareva O, Nevskaya N, Piendl W, Davydova N, Streltsov V, Garber M, Nikonov S, Nucleic Acids Res. 2007;35(21):7389-95. Epub 2007 Oct 25. PMID:17962298 Page seeded by OCA on Sun May 4 11:41:26 2008
