2m0n
From Proteopedia
(Difference between revisions)
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==Solution structure of a DUF3349 annotated protein from Mycobacterium abscessus, MAB_3403c. Seattle Structural Genomics Center for Infectious Disease target MyabA.17112.a.A2== | ==Solution structure of a DUF3349 annotated protein from Mycobacterium abscessus, MAB_3403c. Seattle Structural Genomics Center for Infectious Disease target MyabA.17112.a.A2== | ||
| - | <StructureSection load='2m0n' size='340' side='right' caption='[[2m0n]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2m0n' size='340' side='right'caption='[[2m0n]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2m0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myca9 Myca9]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M0N FirstGlance]. <br> | <table><tr><td colspan='2'>[[2m0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myca9 Myca9]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M0N FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m0n OCA], [http://pdbe.org/2m0n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m0n RCSB], [http://www.ebi.ac.uk/pdbsum/2m0n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2m0n ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m0n OCA], [http://pdbe.org/2m0n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m0n RCSB], [http://www.ebi.ac.uk/pdbsum/2m0n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2m0n ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A protein superfamily with a "Domain of Unknown Function,", DUF3349 (PF11829), is present predominately in Mycobacterium and Rhodococcus bacterial species suggesting that these proteins may have a biological function unique to these bacteria. We previously reported the inaugural structure of a DUF3349 superfamily member, Mycobacterium tuberculosis Rv0543c. Here, we report the structures determined for three additional DUF3349 proteins: Mycobacterium smegmatis MSMEG_1063 and MSMEG_1066 and Mycobacterium abscessus MAB_3403c. Like Rv0543c, the NMR solution structure of MSMEG_1063 revealed a monomeric five alpha-helix bundle with a similar overall topology. Conversely, the crystal structure of MSMEG_1066 revealed a five alpha-helix protein with a strikingly different topology and a tetrameric quaternary structure that was confirmed by size exclusion chromatography. The NMR solution structure of a fourth member of the DUF3349 superfamily, MAB_3403c, with 18 residues missing at the N-terminus, revealed a monomeric alpha-helical protein with a folding topology similar to the three C-terminal helices in the protomer of the MSMEG_1066 tetramer. These structures, together with a GREMLIN-based bioinformatics analysis of the DUF3349 primary amino acid sequences, suggest two subfamilies within the DUF3349 family. The division of the DUF3349 into two distinct subfamilies would have been lost if structure solution had stopped with the first structure in the DUF3349 family, highlighting the insights generated by solving multiple structures within a protein superfamily. Future studies will determine if the structural diversity at the tertiary and quaternary levels in the DUF3349 protein superfamily have functional roles in Mycobacteria and Rhodococcus species with potential implications for structure-based drug discovery. | ||
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| + | Structural diversity in the Mycobacteria DUF3349 superfamily.,Buchko GW, Abendroth J, Robinson JI, Phan IQ, Myler PJ, Edwards TE Protein Sci. 2020 Mar;29(3):670-685. doi: 10.1002/pro.3758. Epub 2019 Nov 21. PMID:31658388<ref>PMID:31658388</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2m0n" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Myca9]] | [[Category: Myca9]] | ||
[[Category: Buchko, G W]] | [[Category: Buchko, G W]] | ||
Revision as of 09:46, 26 February 2020
Solution structure of a DUF3349 annotated protein from Mycobacterium abscessus, MAB_3403c. Seattle Structural Genomics Center for Infectious Disease target MyabA.17112.a.A2
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