2ovw

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[[Image:2ovw.gif|left|200px]]
[[Image:2ovw.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ovw |SIZE=350|CAPTION= <scene name='initialview01'>2ovw</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_2ovw", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=CTA:Catalytic+Residues'>CTA</scene>, <scene name='pdbsite=CTB:Catalytic+Residues'>CTB</scene>, <scene name='pdbsite=CTC:Catalytic+Residues'>CTC</scene> and <scene name='pdbsite=CTD:Catalytic+Residues'>CTD</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CBI:CELLOBIOSE'>CBI</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2ovw| PDB=2ovw | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ovw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ovw OCA], [http://www.ebi.ac.uk/pdbsum/2ovw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ovw RCSB]</span>
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}}
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'''ENDOGLUCANASE I COMPLEXED WITH CELLOBIOSE'''
'''ENDOGLUCANASE I COMPLEXED WITH CELLOBIOSE'''
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[[Category: Schulein, M.]]
[[Category: Schulein, M.]]
[[Category: Sulzenbacher, G.]]
[[Category: Sulzenbacher, G.]]
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[[Category: complexed with cellobiose]]
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[[Category: Complexed with cellobiose]]
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[[Category: endoglucanase i]]
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[[Category: Endoglucanase i]]
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[[Category: glycosyl hydrolase]]
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[[Category: Glycosyl hydrolase]]
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[[Category: glycosylated protein]]
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[[Category: Glycosylated protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:46:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:23:55 2008''
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Revision as of 08:46, 4 May 2008

Template:STRUCTURE 2ovw

ENDOGLUCANASE I COMPLEXED WITH CELLOBIOSE


Overview

The mechanisms involved in the enzymatic degradation of cellulose are of great ecological and commercial importance. The breakdown of cellulose by fungal species is performed by a consortium of free enzymes, known as cellobiohydrolases and endoglucanases, which are found in many of the 57 glycosyl hydrolase families. The structure of the endoglucanase I (EG I), found in glycosyl hydrolase family 7, from the thermophilic fungus Fusarium oxysporum has been solved at 2.3 A resolution. In addition to the native enzyme, structures have also been determined with both the affinity label, 3,4-epoxybutyl beta-D-cellobioside, and the reaction product cellobiose. The affinity label is covalently bound, as expected, to the catalytic nucleophile, Glu197, with clear evidence for binding of both the R and S stereoisomers. Cellobiose is found bound to the -2 and -1 subsites of the enzyme. In marked contrast to the structure of EG I with a nonhydrolyzable thiosaccharide analog, which spanned the -2, -1, and +1 subsites and which had a skew-boat conformation for the -1 subsite sugar [Sulzenbacher, G., et al. (1996) Biochemistry 35, 15280-15287], the cellobiose complex shows no pyranoside ring distortion in the -1 subsite, implying that strain is induced primarily by the additional +1 subsite interactions and that the product is found, as expected, in its unstrained conformation.

About this Structure

2OVW is a Single protein structure of sequence from Fusarium oxysporum. Full crystallographic information is available from OCA.

Reference

Structure of the endoglucanase I from Fusarium oxysporum: native, cellobiose, and 3,4-epoxybutyl beta-D-cellobioside-inhibited forms, at 2.3 A resolution., Sulzenbacher G, Schulein M, Davies GJ, Biochemistry. 1997 May 13;36(19):5902-11. PMID:9153432 Page seeded by OCA on Sun May 4 11:46:22 2008

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