2p1h

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[[Image:2p1h.gif|left|200px]]
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{{Structure
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|GENE= APAF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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{{STRUCTURE_2p1h| PDB=2p1h | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p1h OCA], [http://www.ebi.ac.uk/pdbsum/2p1h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p1h RCSB]</span>
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'''Rapid Folding and Unfolding of Apaf-1 CARD'''
'''Rapid Folding and Unfolding of Apaf-1 CARD'''
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[[Category: Milam, S L.]]
[[Category: Milam, S L.]]
[[Category: Nicely, N I.]]
[[Category: Nicely, N I.]]
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[[Category: apaf-1]]
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[[Category: Apaf-1]]
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[[Category: folding]]
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[[Category: Folding]]
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[[Category: unfolding]]
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[[Category: Unfolding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:08:20 2008''
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Revision as of 09:08, 4 May 2008

Image:2p1h.gif

Template:STRUCTURE 2p1h

Rapid Folding and Unfolding of Apaf-1 CARD


Overview

Caspase recruitment domains (CARDs) are members of the death domain superfamily and contain six antiparallel helices in an alpha-helical Greek key topology. We have examined the equilibrium and kinetic folding of the CARD of Apaf-1 (apoptotic protease activating factor 1), which consists of 97 amino acid residues, at pH 6 and pH 8. The results showed that an apparent two state equilibrium mechanism is not adequate to describe the folding of Apaf-1 CARD at either pH, suggesting the presence of intermediates in equilibrium unfolding. Interestingly, the results showed that the secondary structure is less stable than the tertiary structure, based on the transition mid-points for unfolding. Single mixing and sequential mixing stopped-flow studies showed that Apaf-1 CARD folds and unfolds rapidly and suggest a folding mechanism that contains parallel channels with two unfolded conformations folding to the native conformation. Kinetic simulations show that a slow folding phase is described by a third conformation in the unfolded ensemble that interconverts with one or both unfolded species. Overall, the native ensemble is formed rapidly upon refolding. This is in contrast to other CARDs in which folding appears to be dominated by formation of kinetic traps.

About this Structure

2P1H is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Rapid folding and unfolding of Apaf-1 CARD., Milam SL, Nicely NI, Feeney B, Mattos C, Clark AC, J Mol Biol. 2007 May 25;369(1):290-304. Epub 2007 Mar 15. PMID:17408690 Page seeded by OCA on Sun May 4 12:08:20 2008

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