2p5r
From Proteopedia
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'''Crystal structure of the poplar glutathione peroxidase 5 in the oxidized form''' | '''Crystal structure of the poplar glutathione peroxidase 5 in the oxidized form''' | ||
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[[Category: Rouhier, N.]] | [[Category: Rouhier, N.]] | ||
[[Category: Shawkataly, O.]] | [[Category: Shawkataly, O.]] | ||
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Revision as of 09:25, 4 May 2008
Crystal structure of the poplar glutathione peroxidase 5 in the oxidized form
Overview
Glutathione peroxidases (GPXs) are a group of enzymes that regulate the levels of reactive oxygen species in cells and tissues, and protect them against oxidative damage. Contrary to most of their counterparts in animal cells, the higher plant GPX homologues identified so far possess cysteine instead of selenocysteine in their active site. Interestingly, the plant GPXs are not dependent on glutathione but rather on thioredoxin as their in vitro electron donor. We have determined the crystal structures of the reduced and oxidized form of Populus trichocarpaxdeltoides GPX5 (PtGPX5), using a selenomethionine derivative. PtGPX5 exhibits an overall structure similar to that of the known animal GPXs. PtGPX5 crystallized in the assumed physiological dimeric form, displaying a pseudo ten-stranded beta sheet core. Comparison of both redox structures indicates that a drastic conformational change is necessary to bring the two distant cysteine residues together to form an intramolecular disulfide bond. In addition, a computer model of a complex of PtGPX5 and its in vitro recycling partner thioredoxin h1 is proposed on the basis of the crystal packing of the oxidized form enzyme. A possible role of PtGPX5 as a heavy-metal sink is also discussed.
About this Structure
2P5R is a Single protein structure of sequence from Populus trichocarpa x populus deltoides. Full crystallographic information is available from OCA.
Reference
Crystal structures of a poplar thioredoxin peroxidase that exhibits the structure of glutathione peroxidases: insights into redox-driven conformational changes., Koh CS, Didierjean C, Navrot N, Panjikar S, Mulliert G, Rouhier N, Jacquot JP, Aubry A, Shawkataly O, Corbier C, J Mol Biol. 2007 Jul 13;370(3):512-29. Epub 2007 Apr 19. PMID:17531267 Page seeded by OCA on Sun May 4 12:25:24 2008
