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6adv
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure Analysis of the duplex containing the S2T(2',4'-BNA/LNA)G mismatch pairs== | |
| + | <StructureSection load='6adv' size='340' side='right'caption='[[6adv]], [[Resolution|resolution]] 1.96Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6adv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ADV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ADV FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=9V9:'>9V9</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6adv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6adv OCA], [http://pdbe.org/6adv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6adv RCSB], [http://www.ebi.ac.uk/pdbsum/6adv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6adv ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Oligonucleotides modified with 2'- O,4'- C-spirocyclopropylene-bridged nucleic acid (scpBNA) exhibit excellent duplex-forming ability with their complementary single-stranded RNA (ssRNA). Here, we demonstrate that scpBNA bearing a 2-thiothymine (scpBNA-S(2)T) or 2-selenothymine (scpBNA-Se(2)T) nucleobase provides robust mismatch discrimination capabilities to oligonucleotides without compromising their high binding affinities toward the full complementary ssRNA. X-ray crystallographic analysis of a self-assembling oligonucleotide featuring 2',4'-BNA/LNA-2-thiothymine (2',4'-BNA/LNA-S(2)T, where 2',4'-BNA and LNA stand for "2'- O,4'- C-methylene-bridged nucleic acid" and "locked nucleic acid", respectively), a prototype of scpBNA-S(2)T, revealed that the 2-thiocarbonyl moiety plays a crucial role in the destabilization of thymine-guanine mismatched wobble base pairs. | ||
| - | + | Hybridization and Mismatch Discrimination Abilities of 2',4'-Bridged Nucleic Acids Bearing 2-Thiothymine or 2-Selenothymine Nucleobase.,Habuchi T, Yamaguchi T, Aoyama H, Horiba M, Ito KR, Obika S J Org Chem. 2019 Feb 1;84(3):1430-1439. doi: 10.1021/acs.joc.8b02863. Epub 2019, Jan 24. PMID:30632750<ref>PMID:30632750</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Habuchi, T | + | <div class="pdbe-citations 6adv" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Yamaguchi, T | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Aoyama, H A]] | ||
| + | [[Category: Habuchi, T H]] | ||
| + | [[Category: Obika, S O]] | ||
| + | [[Category: Yamaguchi, T Y]] | ||
| + | [[Category: 2ss']] | ||
| + | [[Category: 4'-bna]] | ||
| + | [[Category: Dna]] | ||
| + | [[Category: Lna]] | ||
| + | [[Category: Mismatched base pair]] | ||
Revision as of 06:31, 27 March 2019
Crystal Structure Analysis of the duplex containing the S2T(2',4'-BNA/LNA)G mismatch pairs
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Categories: Large Structures | Aoyama, H A | Habuchi, T H | Obika, S O | Yamaguchi, T Y | 2ss' | 4'-bna | Dna | Lna | Mismatched base pair
