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2pjr

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Revision as of 06:55, 3 June 2020

HELICASE PRODUCT COMPLEX

Structural highlights

2pjr is a 8 chain structure with sequence from Atcc 12980. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PCRA_GEOSE] DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We have determined two different structures of PcrA DNA helicase complexed with the same single strand tailed DNA duplex, providing snapshots of different steps on the catalytic pathway. One of the structures is of a complex with a nonhydrolyzable analog of ATP and is thus a "substrate" complex. The other structure contains a bound sulphate ion that sits in a position equivalent to that occupied by the phosphate ion produced after ATP hydrolysis, thereby mimicking a "product" complex. In both complexes, the protein is monomeric. Large and distinct conformational changes occur on binding DNA and the nucleotide cofactor. Taken together, these structures provide evidence against an "active rolling" model for helicase action but are instead consistent with an "inchworm" mechanism.

Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism.,Velankar SS, Soultanas P, Dillingham MS, Subramanya HS, Wigley DB Cell. 1999 Apr 2;97(1):75-84. PMID:10199404^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Velankar SS, Soultanas P, Dillingham MS, Subramanya HS, Wigley DB. Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism. Cell. 1999 Apr 2;97(1):75-84. PMID:10199404

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2pjr"

@@ Line 1: / Line 1: @@
 ==HELICASE PRODUCT COMPLEX==
-<StructureSection load='2pjr' size='340' side='right' caption='[[2pjr]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+<StructureSection load='2pjr' size='340' side='right'caption='[[2pjr]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2pjr]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PJR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2pjr]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PJR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2PJR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pjr OCA], [http://pdbe.org/2pjr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pjr RCSB], [http://www.ebi.ac.uk/pdbsum/2pjr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pjr ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2pjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pjr OCA], [http://pdbe.org/2pjr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pjr RCSB], [http://www.ebi.ac.uk/pdbsum/2pjr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pjr ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 31: / Line 31: @@
 ==See Also==
 *[[Helicase|Helicase]]
+*[[Helicase 3D structures|Helicase 3D structures]]
 *[[Molecular Playground/PcrA Helicase|Molecular Playground/PcrA Helicase]]
 *[[PcrA helicase|PcrA helicase]]
@@ Line 38: / Line 39: @@
 </StructureSection>
 [[Category: Atcc 12980]]
+[[Category: Large Structures]]
 [[Category: Dillingham, M S]]
 [[Category: Soultanas, P]]

2pjr

From Proteopedia

Revision as of 06:55, 3 June 2020

HELICASE PRODUCT COMPLEX

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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