2pll

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[[Image:2pll.jpg|left|200px]]
[[Image:2pll.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2pll |SIZE=350|CAPTION= <scene name='initialview01'>2pll</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_2pll", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ABH:2(S)-AMINO-6-BORONOHEXANOIC+ACID'>ABH</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Arginase Arginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.1 3.5.3.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ARG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2pll| PDB=2pll | SCENE= }}
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|RELATEDENTRY=[[2aeb|2AEB]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pll OCA], [http://www.ebi.ac.uk/pdbsum/2pll PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pll RCSB]</span>
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}}
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'''Crystal structure of perdeuterated human arginase I'''
'''Crystal structure of perdeuterated human arginase I'''
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[[Category: Meilleur, F.]]
[[Category: Meilleur, F.]]
[[Category: Moulin, M.]]
[[Category: Moulin, M.]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: perdeuterated protein]]
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[[Category: Perdeuterated protein]]
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[[Category: x-ray structure]]
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[[Category: X-ray structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:22:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:37:39 2008''
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Revision as of 10:22, 4 May 2008

Image:2pll.jpg

Template:STRUCTURE 2pll

Crystal structure of perdeuterated human arginase I


Overview

Arginase is a manganese metalloenzyme that catalyzes the hydrolysis of l-arginine to yield l-ornithine and urea. In order to establish a foundation for future neutron diffraction studies that will provide conclusive structural information regarding proton/deuteron positions in enzyme-inhibitor complexes, we have expressed, purified, assayed, and determined the X-ray crystal structure of perdeuterated (i.e., fully deuterated) human arginase I complexed with 2(S)-amino-6-boronohexanoic acid (ABH) at 1.90A resolution. Prior to the neutron diffraction experiment, it is important to establish that perdeuteration does not cause any unanticipated structural or functional changes. Accordingly, we find that perdeuterated human arginase I exhibits catalytic activity essentially identical to that of the unlabeled enzyme. Additionally, the structure of the perdeuterated human arginase I-ABH complex is identical to that of the corresponding complex with the unlabeled enzyme. Therefore, we conclude that crystals of the perdeuterated human arginase I-ABH complex are suitable for neutron crystallographic study.

About this Structure

2PLL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Expression, purification, assay, and crystal structure of perdeuterated human arginase I., Di Costanzo L, Moulin M, Haertlein M, Meilleur F, Christianson DW, Arch Biochem Biophys. 2007 Sep 1;465(1):82-9. Epub 2007 May 21. PMID:17562323 Page seeded by OCA on Sun May 4 13:22:15 2008

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